Isothermal real-time polymerase chain reaction detection of Herpes Simplex Virus Type 1 on a light-actuated digital microfludics platform

机译：在光驱动数字微流控平台上等温实时聚合酶链反应检测1型单纯疱疹病毒

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

We report on a light-actuated digital microfluidics platform for real-time, isothermal helicase-dependent polymerase chain reaction (PCR). This platform is used to detect Herpes Simplex Virus Type 1 (HSV-1). Reagents in droplets are individually addressed, mixed, transported and arrayed on-chip before the PCR process. 16 droplets were formed from mixing 400 nl PCR master mix droplets with 35 nl HSV-1 viral lysate droplets, arrayed into a 4×4 matrix, and amplified simultaneously, all on chip. Amplification and detection of HSV-1 can be achieved within 45 minutes. No cross contamination was observed.

机译：我们报告的实时，等温解旋酶依赖的聚合酶链反应（PCR）的光驱动数字微流控平台。该平台用于检测1型单纯疱疹病毒（HSV-1）。在PCR过程之前，对液滴中的试剂进行单独寻址，混合，运输和在芯片上排列。通过将400 nl PCR主混合物液滴与35 nl HSV-1病毒裂解液液滴混合，形成16个液滴，排列成4×4矩阵，并同时扩增，全部在芯片上。 HSV-1的扩增和检测可以在45分钟内完成。没有观察到交叉污染。

著录项

来源
《International Conference on Solid-State Sensors, Actuators and Microsystems》|2013年|2791-2794|共4页
会议地点
作者
Pei Shao Ning; Wang Yi-Lun; Lin Chih-Ting; Wu Ming C.;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类
关键词
Digital Microfluidics; EWOD; Electrowetting-on-Dielectric; Herpes Simplex Virus; Isothermal Polymerase Chain Reaction; Light-Actuated Digital Microfluidics; Polymerase Chain Reaction;

机译：数字微流控; EWOD;介电电泳;单纯疱疹病毒;等温聚合酶链反应;光致数字微流控;聚合酶链反应;

相似文献

外文文献
中文文献
专利

1. Duplex Real-Time Polymerase Chain Reaction Assay for Detection and Quantification of Herpes Simplex Virus Type 1 and Herpes Simplex Virus Type 2 in Genital and Cutaneous Lesions. [J] . FilEn F, Allard A, Herrmann B Sexually transmitted diseases . 2004,第6期

机译：在生殖器和皮肤病变中检测和定量1型单纯疱疹病毒和2型单纯疱疹病毒的双工实时聚合酶链反应测定法。
2. Clinical Validation of a New Triplex Real-Time Polymerase Chain Reaction Assay for the Detection and Discrimination of Herpes simplex Virus Types 1 and 2. [J] . Reil H, Bartlime A, Drerup J, The Journal of molecular diagnostics: JMD . 2008,第4期

机译：一种用于检测和区分1型和2型单纯疱疹病毒的新型三重实时聚合酶链反应测定的临床验证。
3. Detection of varicella-zoster virus DNA in 414 human trigeminal ganglia from cadavers by the polymerase chain reaction: a comparison of the detection rate of varicella-zoster virus and herpes simplex virus type 1. [J] . Inoue H, Motani Saitoh H, Sakurada K, Journal of Medical Virology . 2010,第2期

机译：聚合酶链反应检测尸体中414人三叉神经节中的水痘带状疱疹病毒DNA：水痘带状疱疹病毒和1型单纯疱疹病毒检出率的比较。
4. ISOTHERMAL REAL-TIME POLYMERASE CHAIN REACTION DETECTION OF HERPES SIMPLEX VIRUS TYPE 1 ON A LIGHT-ACTUATED DIGITAL MICROFLUDICS PLATFORM [C] . Shao Ning Pei, Yi-Lun Wang, Chih-Ting Lin, International Conference on Solid-State Sensors, Actuators and Microsystems . 2013

机译：等温实时聚合酶链反应检测单纯疱疹病毒型1型在光致动的数字微氟化物平台上
5. Associations between herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), cytomegalovirus (CMV), Epstein Barr virus (EBV), human papilloma virus (HPV), Chlamydia trachomatis, Neisseria gonorrhoeae infections and preeclampsia. [D] . Rustveld, Luis Orlando. 2005

机译：1型和2型单纯疱疹病毒（HSV-1和HSV-2），巨细胞病毒（CMV），爱泼斯坦巴尔病毒（EBV），人乳头瘤病毒（HPV），沙眼衣原体，淋病奈瑟氏球菌感染和先兆子痫之间的关联。
6. Clinical Validation of a New Triplex Real-Time Polymerase Chain Reaction Assay for the Detection and Discrimination of Herpes simplex Virus Types 1 and 2 [O] . Heide Reil, Ariane Bartlime, Jana Drerup, 2008

机译：一种新的三重实时聚合酶链反应检测和鉴别1型和2型单纯疱疹病毒的临床验证
7. Clinical Validation of a New Triplex Real-Time Polymerase Chain Reaction Assay for the Detection and Discrimination of Herpes simplex Virus Types 1 and 2 [O] . Reil, Heide, Bartlime, Ariane, Drerup, Jana, 2008

机译：一种新的三重实时聚合酶链反应检测和鉴别1型和2型单纯疱疹病毒的临床验证
8. Amplification of Herpes Simplex Virus Types 1 and 2 and Human Herpes Virus Type 5 Polymerase Gene Segment From Formalin-Fixed Brain Tissue From Alzheimer's Disease Patients [R] . Henrichs, L. E. 2005

机译：从阿尔茨海默病患者的福尔马林固定脑组织扩增1型和2型单纯疱疹病毒和人类疱疹病毒5型聚合酶基因片段

Isothermal real-time polymerase chain reaction detection of Herpes Simplex Virus Type 1 on a light-actuated digital microfludics platform

摘要

著录项

相似文献

相关主题

期刊订阅