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首页> 外文期刊>Sexually transmitted diseases >Duplex Real-Time Polymerase Chain Reaction Assay for Detection and Quantification of Herpes Simplex Virus Type 1 and Herpes Simplex Virus Type 2 in Genital and Cutaneous Lesions.
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Duplex Real-Time Polymerase Chain Reaction Assay for Detection and Quantification of Herpes Simplex Virus Type 1 and Herpes Simplex Virus Type 2 in Genital and Cutaneous Lesions.

机译：在生殖器和皮肤病变中检测和定量1型单纯疱疹病毒和2型单纯疱疹病毒的双工实时聚合酶链反应测定法。

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BACKGROUND:: A sensitive and specific method for detecting herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) is important for diagnosing genital and cutaneous infections. GOAL:: The goal of this study was to compare quantitative real-time polymerase chain reaction (qPCR) with virus culture for diagnosis of genital and cutaneous HSV-1 and HSV-2. STUDY DESIGN:: A duplex qPCR system for quantification of DNA from HSV-1 and HSV-2 was developed. Duplicate swabs for PCR and virus culture were collected from 89 patients attending our sexually transmitted infection and dermatology clinic. RESULTS:: The duplex qPCR had a linear measure interval of 10-10 copies/mL. The detection limit was between 1 and 5 copies per reaction. qPCR detected HSV in 57 (64%) specimens and virus was isolated in 45 (50%) cases. First-episode infections showed higher viral quantities with a median value of 4.2 x 10 copies per reaction compared with recurrent infections with 1.0 x 10 (P = 0.0002). HSV-1 was more likely to be the cause of first-episode genital infections (72%), and HSV-2 of recurrent and atypical genital manifestations (73%). CONCLUSION:: Real-time PCR is a sensitive method for diagnosing genital herpes, and the duplex format is convenient for typing. The method increased the detection rate by 27% compared with virus culture.
机译：背景：一种灵敏，特异的方法来检测1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）对于诊断生殖器和皮肤感染很重要。目标：该研究的目的是将定量实时聚合酶链反应（qPCR）与病毒培养进行比较，以诊断生殖器和皮肤HSV-1和HSV-2。研究设计：开发了用于定量HSV-1和HSV-2 DNA的双链qPCR系统。从我们性传播感染和皮肤病学诊所的89例患者中收集了用于PCR和病毒培养的双拭子。结果：双链qPCR的线性测量区间为10-10个拷贝/ mL。每个反应的检出限为1-5个拷贝。 qPCR在57个样本（64％）中检测到HSV，在45个样本（50％）中分离出病毒。首发感染的病毒数量更高，每个反应的中位数为4.2 x 10份，而复发感染的病毒数量为1.0 x 10（P = 0.0002）。 HSV-1更有可能是首发生殖器感染的原因（72％），而HSV-2是复发性和非典型生殖器表现的原因（73％）。结论：实时荧光定量PCR是一种诊断生殖器疱疹的灵敏方法，双工格式便于打字。与病毒培养相比，该方法的检测率提高了27％。

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