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首页> 外文期刊>Journal de mycologie medicale >Optimisation of process parameters for growth and bioactive metabolite produced by a salt-tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D
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Optimisation of process parameters for growth and bioactive metabolite produced by a salt-tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D

机译：耐盐和嗜碱放线菌，Streptomyces tanashiensis菌株A2D的生长和生物活性代谢物的工艺参数的优化

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摘要

An alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D isolated from soil of phoomdi in Loktak Lake of Manipur, India exhibited optimum growth at 2% (w/v) NaCl and pH 8. It is Gram-positive filamentous bacterium which exhibits a broad spectrum of antimicrobial activity against fungi and bacteria. Optimum salt and pH for antibiotic production were 2% (w/v) and 8 respectively. Studies on nutritional factors revealed that highest antibiotic production was obtained when glucose and soyabean meal at 1% (w/v) were used as carbon and nitrogen sources respectively. Seven days of incubation at 28 degC. was found to be the optimum for antibiotic production by the strain. The antimicrobial agent produced by the strain was extracted with ethyl acetate as solvent and purified by TLC. The purified active compound with lambda_(max) 241.99 nm has got the lowest minimum inhibitory concentration (MIC) against Staphylococcus aureus (50 mug/ml), whereas highest (100 mug/ml) was recorded against Candida albicans and Fusarium moniliform. So far only few alkaliphilic actinomycetes have been explored for their antimicrobial potential.
机译：从印度曼尼普尔邦Loktak湖的phoomdi土壤中分离出的一种嗜碱放线菌，Streptomyces tanashiensis菌株A2D在2％（w / v）NaCl和pH 8的条件下表现出最佳生长。它是革兰氏阳性丝状细菌，具有广泛的抗菌谱对抗真菌和细菌的活性。抗生素生产的最佳盐和pH分别为2％（w / v）和8。营养因素研究表明，将1％（w / v）的葡萄糖和大豆粉分别用作碳和氮源时，可获得最高的抗生素产量。在28摄氏度下孵育7天。被发现是该菌株生产抗生素的最佳方法。用乙酸乙酯作为溶剂提取菌株产生的抗菌剂，并通过TLC纯化。纯化的具有λ（最大）241.99 nm的活性化合物对金黄色葡萄球菌的最低抑菌浓度（MIC）最低（50杯/毫升），而对白色念珠菌和镰刀菌的最高抑制浓度最高（100杯/毫升）。迄今为止，只有极少数的嗜碱放线菌具有抗菌潜力。

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