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首页> 外文期刊>Joint, bone, spine : >Pro-inflammatory effect of leptin on peripheral blood mononuclear cells of patients with ankylosing spondylitis.
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Pro-inflammatory effect of leptin on peripheral blood mononuclear cells of patients with ankylosing spondylitis.

机译：瘦素对强直性脊柱炎患者外周血单个核细胞的促炎作用。

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OBJECTIVES: This study was performed to investigate whether leptin mRNA expression is increased from peripheral blood mononuclear cells (PBMCs) of patients with active ankylosing spondylitis (AS) and whether it stimulates the production of pro-inflammatory cytokines. METHODS: Twenty patients with active AS were enrolled and their Bath AS disease activity index (BASDAI) and levels of acute phase reactants were measured. Leptin, interleukin (IL)-6, and tumor necrosis factor (TNF)-alpha mRNA expressions and their protein productions were determined in PBMCs of patients with AS using semi-quantitative reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay. Then, the results were compared with those from 20 healthy controls, as were clinical and laboratory parameters reflecting disease activity. The changes of pro-inflammatory cytokine productions from PBMCs in response to leptin stimulation were also determined. RESULTS: Leptin, IL-6 and TNF-alpha mRNA expressions of PMBCs from patients with AS were significantly higher than controls. Similar significances were also found in the measurements for leptin and cytokine levels of supernatants, and leptin levels correlated well with IL-6 expression (r=0.871, p<0.001) and BASDAI (r=0.691, p<0.001) in patients with AS. Stimulation of PBMCs by exogenous leptin significantly increased the production of IL-6 and TNF-alpha in PBMCs from patients with AS in a dose-dependent fashion and these increases were much exacerbated compared to controls. CONCLUSION: Our results shows that leptin production is increased and its stimulation of PBMCs significantly increased the production of pro-inflammatory cytokines in patients with active AS, suggesting its pro-inflammatory effect in pathogenesis of AS.
机译：目的：本研究旨在研究活动性强直性脊柱炎（AS）患者外周血单个核细胞（PBMC）瘦素mRNA表达是否增加以及是否刺激促炎性细胞因子的产生。方法：纳入20例活动性AS患者，并测量其Bath AS疾病活动指数（BASDAI）和急性期反应物水平。使用半定量逆转录酶-聚合酶链反应和酶联免疫吸附测定法测定AS患者的PBMC中的瘦素，白介素（IL）-6和肿瘤坏死因子（TNF）-αmRNA表达及其蛋白产生。然后，将结果与20位健康对照者的结果进行比较，并与反映疾病活动的临床和实验室参数进行比较。还确定了响应瘦素刺激的PBMC促炎细胞因子产生的变化。结果：AS患者PMBCs的瘦素，IL-6和TNF-αmRNA表达明显高于对照组。在上皮细胞瘦素和细胞因子水平的测量中也发现了相似的意义，并且瘦素水平与AS患者的IL-6表达（r = 0.871，p <0.001）和BASDAI（r = 0.691，p <0.001）密切相关。 。外源瘦素刺激PBMC以剂量依赖的方式显着增加了AS患者的PBMC中IL-6和TNF-α的产生，与对照组相比，这些增加更为严重。结论：我们的结果表明瘦素的产生增加，其对PBMC的刺激显着增加了活动性AS患者的促炎细胞因子的产生，表明其在AS的发病机理中具有促炎作用。

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