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Rapid Detection of Pathogenic Bacteria from Fresh Produce by Filtration and Surface-Enhanced Raman Spectroscopy

机译:过滤和表面增强拉曼光谱法快速检测新鲜农产品中的致病细菌

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摘要

The detection of Salmonella Poona from cantaloupe cubes and E. coli O157:H7 from lettuce has been explored by using a filtration method and surface-enhanced Raman spectroscopy (SERS) based on vancomycin-functionalized silver nanorod array substrates. It is found that with a two-step filtration process, the limit of detection (LOD) of Salmonella Poona from cantaloupe cubes can be as low as 100 CFU/mL in less than 4 h, whereas the chlorophyll in the lettuce causes severe SERS spectral interference. To improve the LOD of lettuce, a three-step filtration method with a hydrophobic filter is proposed. The hydrophobic filter can effectively eliminate the interferences from chlorophyll and achieve a LOD of 1000 CFU/mL detection of E. coli O157:H7 from lettuce samples within 5 h. With the low LODs and rapid detection time, the SERS biosensing platform has demonstrated its potential as a rapid, simple, and inexpensive means for pathogenic bacteria detection from fresh produce.
机译:基于万古霉素功能化的银纳米棒阵列底物,使用过滤方法和表面增强拉曼光谱(SERS),探索了从哈密瓜立方体中检出沙门氏菌沙门氏菌和从生菜中检出大肠杆菌O157:H7的探索。发现采用两步过滤过程,在不到4小时的时间内,哈密瓜立方体沙门氏菌Poona的检出限(LOD)可能低至100 CFU / mL,而生菜中的叶绿素会导致严重的SERS光谱干扰。为了提高生菜的LOD,提出了一种采用疏水过滤器的三步过滤方法。疏水过滤器可有效消除叶绿素的干扰,并在5小时内从生菜样品中检出大肠杆菌O157:H7的LOD为1000 CFU / mL。 SERS生物传感平台具有较低的检测限和快速的检测时间,已证明其具有潜力,可以快速,简便,廉价地检测新鲜农产品中的致病细菌。

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