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首页> 外文期刊>Journal of Analytical Toxicology >Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood.
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Solid-phase extraction and gas chromatographic- mass spectrometric determination of the veterinary drug xylazine in human blood.

机译：固相萃取和气相色谱-质谱法测定人血中的兽药甲苯噻嗪。

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摘要

This paper presents a method for the determination of xylazine in whole blood using solid-phase extraction and gas chromatography-mass spectrometry. This technique required only 0.5 mL of sample, and protriptyline was used as internal standard (IS). Limits of detection and quantitation (LOQ) were 2 and 10 ng/mL, respectively. The method was found to be linear between the LOQ and 3.50 microg/mL, with correlation coefficients higher than 0.9922. Precision (intra- and interday) and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The analyte was stable in the matrix for at least 18 h at room temperature and for at least three freeze/thaw cycles. Mean recovery, calculated at three concentration levels, was 87%. To the best of our knowledge, this is the first time that solid-phase extraction is used as sample preparation technique for the determination of this compound in biological media. Because of its simplicity and speed when compared to other extraction techniques, the herein described method can be successfully applied in the diagnosis of intoxications by xylazine.
机译：本文提出了一种固相萃取-气相色谱-质谱法测定全血中甲苯噻嗪的方法。该技术仅需0.5 mL样品，而普罗替林被用作内标（IS）。检测限和定量限（LOQ）分别为2和10 ng / mL。发现该方法在LOQ和3.50 microg / mL之间呈线性，相关系数高于0.9922。精确度（日内和日间）和准确度均符合生物分析方法验证中通常接受的标准。在室温下，分析物在基质中稳定至少18 h，并且至少进行三个冷冻/解冻循环。在三个浓度水平下计算的平均回收率为87％。据我们所知，这是首次将固相萃取用作样品制备技术来测定生物介质中的该化合物。由于与其他提取技术相比其简单性和速度快，本文描述的方法可成功地用于甲苯噻嗪的中毒诊断。

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