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首页> 外文期刊>Journal of Anatomy >The survival of neural precursor cell grafts is influenced by in vitro expansion.
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The survival of neural precursor cell grafts is influenced by in vitro expansion.

机译：神经前体细胞移植物的存活受到体外扩增的影响。

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Embryonic neural precursor cells (ENPs) provide a potential alternative for transplantation in neurodegenerative diseases, as they can be expanded in culture, avoiding many of the practical obstacles that limit the application of transplanting primary neurones. However, grafts of ENPs into animal models show variable survival and limited differentiation into neurones. The effect of expansion time on their ability to survive and differentiate may be an important factor in this and has not been examined directly. In these experiments, murine and human ENPs were expanded for short (4 weeks) and long (20 weeks) periods before transplantation into the adult rat striatum. Whereas grafts of both short- and long-term expanded human ENPs survived for 4 weeks following transplantation, by 20 weeks all long-term expanded grafts had disappeared. Murine ENPs behaved similarly: only grafts of short-term expanded ENPs survived at 12 weeks following transplantation. RT-PCR analysis of ENP cultures after 4 and 20 weeksof expansion demonstrated changes in expression of a number of different groups of genes. We conclude that long-term expansion of ENPs profoundly impairs their ability to survive long-term after transplantation into the adult brain. This has implications for the potential use of these cells for neural transplantation strategies.
机译：胚胎神经前体细胞（ENP）为神经退行性疾病的移植提供了潜在的替代方法，因为它们可以在培养物中扩增，避免了许多限制移植原代神经元应用的实际障碍。然而，ENPs移植到动物模型中显示出可变的生存和有限的分化成神经元。扩展时间对其生存和分化能力的影响可能是其中一个重要因素，尚未进行直接检查。在这些实验中，将鼠和人的ENP短时间（4周）和长时间（20周）扩增，然后再移植到成年大鼠纹状体中。短期和长期扩增的人类ENPs移植物在移植后可存活4周，而到20周时，所有长期扩增的移植物都消失了。鼠类ENP的行为相似：仅短期扩展的ENP移植物在移植后12周存活。扩增4周和20周后，ENP培养物的RT-PCR分析表明，许多不同基因组的表达都有变化。我们得出的结论是，ENPs的长期扩展严重损害了它们在移植到成人大脑后长期生存的能力。这暗示了这些细胞在神经移植策略中的潜在用途。

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