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Untangling Autophagy Measurements All Fluxed Up

机译:杂乱无章的自噬测量结果

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摘要

Autophagy is an important physiological process in the heart, and alterations in autophagic activity can exacerbate or mitigate injury during various pathological processes. Methods to assess autophagy have changed rapidly because the field of research has expanded. As with any new field, methods and standards for data analysis and interpretation evolve as investigators acquire experience and insight. The purpose of this review is to summarize current methods to measure autophagy, selective mitochondrial autophagy (mitophagy), and autophagic flux. We will examine several published studies where confusion arose in data interpretation, to illustrate the challenges. Finally, we will discuss methods to assess autophagy in vivo and in patients.
机译:自噬是心脏中重要的生理过程,自噬活动的改变会加剧或减轻各种病理过程中的损伤。由于研究领域的扩展,评估自噬的方法已迅速改变。与任何新领域一样,随着研究人员获得经验和见识,数据分析和解释的方法和标准也在不断发展。这篇综述的目的是总结当前测量自噬,选择性线粒体自噬(有丝分裂)和自噬通量的方法。我们将研究数个已发表的研究,这些研究在数据解释中引起混淆,以说明挑战。最后,我们将讨论评估体内和患者自噬的方法。

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