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首页> 外文期刊>Journal of Animal Science >Oral administration of putrescine and proline during the suckling period improves epithelial restitution after early weaning in piglets
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Oral administration of putrescine and proline during the suckling period improves epithelial restitution after early weaning in piglets

机译：哺乳期口服腐胺和脯氨酸可改善仔猪早期断奶后的上皮恢复

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摘要

Polyamines are necessary for normal integrity and the restitution after injury of the gastrointestinal epithelium. The objective of this study was to investigate the effects of oral administration of putrescine and proline during the suckling period on epithelial restitution after early weaning in piglets. Eighteen neonatal piglets (Duroc x Landrace x Large Yorkshire) from 3 litters (6 piglets per litter) were assigned to 3 groups, representing oral administration with an equal volume of saline (control), putrescine (5 mg/kg BW), and proline (25 mg/kg BW) twice daily from d 1 to weaning at 14 d of age. Plasma and intestinal samples were obtained 3 d after weaning. The results showed that oral administration of putrescine or proline increased the final BW and ADG of piglets compared with the control (P 0.05). Proline treatment decreased plasma D-lactate concentration but increased the villus height in the jejunum and ileum, as well as the percentage of proliferating cell nuclear antigen (PCNA) positive cells and alkaline phosphatase (AKP) activity in the jejunal mucosa (P 0.05). The protein expressions for zonula occludens (ZO-1), occludin, and claudin-3 (P 0.05) but not mRNA were increased in the jejunum of putrescine-and proline-treated piglets compared with those of control piglets. The voltage-gated K+ channel (Kv) 1.1 protein expression in the jejunum of piglets administrated with putrescine and the Kv1.5 mRNA and Kv1.1 protein levels in the ileum of piglets administrated with proline were greater than those in control piglets (P 0.05). These findings indicate that polyamine or its precursor could improve mucosal proliferation, intestinal morphology, as well as tight junction and potassium channel protein expressions in early-weaned piglets, with implications for epithelial restitution and barrier function after stress injury.
机译：多胺对于正常完整性和胃肠道上皮损伤后的恢复是必需的。这项研究的目的是调查仔猪早期断奶后哺乳期口服腐胺和脯氨酸对上皮修复的影响。将来自3窝（每窝6头小猪）的18头新生仔猪（Duroc x Landrace x大约克郡）分为3组，分别口服等量的生理盐水（对照组），腐胺（5 mg / kg体重）和脯氨酸（25 mg / kg BW）从d 1开始每天两次，至14 d断奶。断奶3天后取血浆和肠标本。结果表明，与对照组相比，口服腐胺或脯氨酸可增加仔猪的最终体重和日增重（P <0.05）。脯氨酸治疗降低血浆D-乳酸浓度，但增加空肠和回肠的绒毛高度，以及空肠黏膜中增殖细胞核抗原（PCNA）阳性细胞百分比和碱性磷酸酶（AKP）活性（P <0.05） 。与对照仔猪相比，在腐胺和脯氨酸处理的仔猪的空肠中，小带闭合蛋白（ZO-1），闭合蛋白和claudin-3的蛋白表达增加（P <0.05），但mRNA没有增加。腐胺给药仔猪空肠中电压门控的K +通道（Kv）1.1蛋白表达，脯氨酸给药仔猪回肠Kv1.5 mRNA和Kv1.1蛋白水平高于对照组仔猪（P < 0.05）。这些发现表明，多胺或其前体可以改善断奶仔猪的粘膜增殖，肠道形态以及紧密连接和钾通道蛋白的表达，对应激损伤后的上皮恢复和屏障功能有影响。

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