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首页> 外文期刊>Journal of Animal Science >Impact of mash feeding versus pellets on propionic/butyric acid levels and on total Escherichia coli load in the gastrointestinal tract of growing pigs
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Impact of mash feeding versus pellets on propionic/butyric acid levels and on total Escherichia coli load in the gastrointestinal tract of growing pigs

机译：饲料与颗粒饲料对生长猪的丙酸/丁酸水平以及胃肠道大肠杆菌总负荷的影响

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Feed characteristics may influence the bacterial community composition and metabolic activities in the pig gastrointestinal tract, known to be associated with positive effects on the gut. Use of mash feed is associated with reduced Salmonella excretion, but little is known of its effect on the Escherichia coli population or of the mechanism of action. Our objectives were to assess the effect of feed texture combined with feed particle size on VFA profiles and levels, total E. coli count, and the presence of genes encoding virulence factors of pathogenic E. coli strains in the digestive tract along with their impact on pig performance of fattening pigs. Pigs (n = 840) on a commercial farm received mash or pellet diets of different particle sizes during the fattening period. Caecal and colon contents from 164 pigs were sampled at the slaughterhouse for enumeration of E. coli by quantitative PCR (qPCR) and for VFA quantification by capillary gas chromatography. The yccT gene was used to enumerate total E. coli. Improved pig performances associated with pellet texture and a 500-mu m size were observed. Caecal (P = 0.02) and colon (P < 0.01) propionic acid concentrations were lower for pigs receiving pellet rather than mash feed. Similarly, caecal (P = 0.01) and colon (P < 0.001) butyric acid concentrations were also lower for pigs receiving pellet rather than mash feed, as determined by capillary gas chromatography. Moreover, caecal (P = 0.03) and colon (P < 0.001) butyric acid concentrations were higher for pigs receiving a feed with a 1,250-mu m particle size rather than a 500-mu m particle size. On the other hand, total caecal and colon E. coli levels were higher for pigs receiving pellet feed than for those receiving mash feed. For total E. coli enumeration, caecal (P < 0.01) and colon (P < 0.01) yccT gene copies were higher for pigs receiving pellet rather than mash feed. No effect of particle size on fatty acid concentrations or on E. coli numbers was observed. Virulence gene quantification revealed no trend. Taken together, results showed that mash feed is associated with lower growth performance but with favorable intestinal changes linked to VFA levels and E. coli reduction in the intestine.
机译：饲料特性可能会影响猪胃肠道的细菌群落组成和代谢活性，已知与肠道的积极影响有关。饲料的使用与沙门氏菌的排泄减少有关，但对它对大肠杆菌种群的影响或作用机理知之甚少。我们的目标是评估饲料质地，饲料粒度对VFA概况和水平，总大肠杆菌数以及在消化道中编码致病性大肠杆菌菌株毒力因子的基因的影响及其对饲料的影响。猪的育肥性能。在育肥期间，一个商业农场的猪（n = 840）接受了不同粒径的土豆泥或颗粒饲料。在屠宰场采样来自164头猪的盲肠和结肠内容物，通过定量PCR（qPCR）进行大肠杆菌计数，并通过毛细管气相色谱法进行VFA定量。 yccT基因用于枚举总大肠杆菌。观察到与颗粒质地和500微米大小有关的生猪性能得到改善。接受颗粒饲料而不是饲料的猪的盲肠（P = 0.02）和结肠（P <0.01）丙酸浓度较低。同样，通过毛细管气相色谱法测定，接受颗粒饲料而不是饲料的猪的盲肠（P = 0.01）和结肠（P <0.001）丁酸浓度也较低。此外，接受1250微米粒径而非500微米粒径饲料的猪的盲肠（P = 0.03）和结肠（P <0.001）丁酸浓度更高。另一方面，接受颗粒饲料的猪的总盲肠和结肠大肠杆菌水平要高于接受饲料的猪。对于总的大肠杆菌计数，接受颗粒饲料而不是饲料的猪的盲肠（P <0.01）和结肠（P <0.01）yccT基因拷贝数更高。没有观察到粒径对脂肪酸浓度或大肠杆菌数量的影响。毒力基因定量无趋势。两者合计，结果表明饲料与较低的生长性能有关，但与肠道中VFA水平和大肠杆菌减少相关的有利的肠道变化有关。

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