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首页> 外文期刊>Japanese Journal of Cancer Research >In vitro induction of cytotoxic T lymphocytes against HTLV-I-infected T-cells from adult T-cell leukemia patients, asymptomatic HTLV-I carriers and seronegative healthy donors.
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In vitro induction of cytotoxic T lymphocytes against HTLV-I-infected T-cells from adult T-cell leukemia patients, asymptomatic HTLV-I carriers and seronegative healthy donors.

机译：针对来自成人T细胞白血病患者，无症状HTLV-1携带者和血清阴性健康供者的HTLV-1感染的T细胞的细胞毒性T淋巴细胞的体外诱导。

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摘要

We investigated an in vitro method to produce cytotoxic T lymphocytes (CTLs) against HTLV-I-infected T-cells using peripheral blood mononuclear cells (PBMC) of adult T-cell leukemia (ATL) patients, asymptomatic HTLV-I carriers (AC) and seronegative healthy donors. The PBMC were restimulated repeatedly for 4 weeks with HLA-matched HTLV-I-infected T-cells which had been pretreated at 56 degrees C for 30 min to inactivate infectious HTLV-I. The culture medium included 10-100 units/ml of recombinant lymphokines (rIL-1, rIL-2, rIL-4, rIL-6 and rIL-7) and 10% fetal calf serum in RPMI-1640 medium. The cytotoxic activity was measured against HLA-matched HTLV-I-infected T-cell lines after CD4+ or CD8+ cells were positively panned from the cultured PBMC. The PBMC of ATL, AC and healthy donors were able to produce either CD4+ or CD8+ CTLs against HTLV-I-related antigens (env, gag, p21x, p27rex and p40tax) as well as the antigen(s) of as-yet unknown specificity expressed on HTLV-I-infected T-cells. All the CTLs recognized the specific antigens in the context of either class I or class II HLA types. These results indicated that ATL patients, AC and healthy donors were immunocompetent to generate CTLs against HTLV-I-infected T-cells and probably against HTLV-I-transformed T-cells.
机译：我们研究了使用成人T细胞白血病（ATL）患者的外周血单核细胞（PBMC），无症状HTLV-1携带者（AC）产生针对HTLV-I感染的T细胞的细胞毒性T淋巴细胞（CTL）的体外方法和血清阴性的健康捐献者。 PBMC用HLA匹配的HTLV-I感染的T细胞重复再刺激4周，该T细胞已在56摄氏度下预处理了30分钟以灭活感染性HTLV-1。培养基在RPMI-1640培养基中包含10-100单位/ ml的重组淋巴因子（rIL-1，rIL-2，rIL-4，rIL-6和rIL-7）和10％的胎牛血清。从培养的PBMC中阳性淘选CD4 +或CD8 +细胞后，测定其对HLA匹配的HTLV-1感染的T细胞系的细胞毒活性。 ATL，AC和健康捐献者的PBMC能够产生针对HTLV-1相关抗原（env，gag，p21x，p27rex和p40tax）以及迄今未知特异性的抗原的CD4 +或CD8 + CTL。在HTLV-1感染的T细胞上表达。所有的CTL在I类或II类HLA类型的背景下均识别特定抗原。这些结果表明，ATL患者，AC和健康供体具有免疫能力以产生针对HTLV-1感染的T细胞并且可能针对HTLV-1转化的T细胞的CTL。

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