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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Isoflavone-free soy protein prepared by column chromatography reduces plasma cholesterol in rats.
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Isoflavone-free soy protein prepared by column chromatography reduces plasma cholesterol in rats.

机译：通过柱色谱法制备的不含异黄酮的大豆蛋白可降低大鼠的血浆胆固醇。

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摘要

To know whether isoflavones are responsible for the hypocholesterolemic effect of soy protein, the effect on plasma cholesterol of isoflavone-free soy protein prepared by column chromatography was examined in rats. Five-week-old male Sprague-Dawley rats were fed cholesterol-enriched AIN-93G diets containing either 20% casein (CAS), 20% soy protein isolate (SPI), 20% isoflavone-free SPI (IF-SPI), 19.7% IF-SPI + 0.3% isoflavone-rich fraction (isoflavone concentrate, IC), or 20% CAS + 0.3% IC for 2 weeks. Plasma total cholesterol concentrations of rats fed SPI and IF-SPI were comparable and were significantly lower than that of rats fed CAS. The addition of IC to the CAS and IF-SPI did not influence plasma cholesterol level. Fecal steroid excretion of the three SPI groups was higher than that of the two CAS groups, whereas the addition of IC showed no effect. Thus, a significant fraction of the cholesterol-lowering effect of SPI in rats can be attributed to the protein content, but the isoflavones and other minor constituents may also play a role.
机译：为了了解异黄酮是否对大豆蛋白的降胆固醇作用负责，在大鼠中检查了通过柱色谱法制备的不含异黄酮的大豆蛋白对血浆胆固醇的影响。给五周大的Sprague-Dawley雄性大鼠喂食富含胆固醇的AIN-93G日粮，其中含有20％酪蛋白（CAS），20％大豆分离蛋白（SPI），20％不含异黄酮的SPI（IF-SPI），19.7 ％IF-SPI + 0.3％富含异黄酮的馏分（异黄酮浓缩物，IC），或20％CAS + 0.3％IC，持续2周。喂食SPI和IF-SPI的大鼠血浆总胆固醇浓度相当，并且显着低于喂食CAS的大鼠。在CAS和IF-SPI中添加IC不会影响血浆胆固醇水平。三个SPI组的粪便类固醇排泄量高于两个CAS组，而添加IC则无作用。因此，大鼠SPI降低胆固醇的作用的很大一部分可以归因于蛋白质含量，但是异黄酮和其他次要成分也可能起作用。

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