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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Resistance of soybean vegetative storage proteins (S-VSPs) to proteolysis by rumen microorganisms
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Resistance of soybean vegetative storage proteins (S-VSPs) to proteolysis by rumen microorganisms

机译：大豆营养储藏蛋白（S-VSPs）对瘤胃微生物蛋白水解的抵抗力

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摘要

Soybean vegetative storage proteins (S-VSPs) are lysine-rich and, hence, are potentially of high nutritive value for high productive ruminants. Using S-VSPs from wild-type soybean and from transgenic tobacco plants expressing either one of the two S-VSPs subunits (S-VSPalpha or S-VSbeta) or both, we tested their stability in cow rumen fluid under in situ conditions, using SIDS-polyacrylamide gel electrophoresis. Proteolysis and degradation pattern of S-VSPs from transgenic tobacco leaves occurred relatively fast compared with that of wild-type (WT) soybean plants. Comparing the two S-VSPs subunits expressed in transgenic plants, we found that S-VSPalpha was degraded much faster than S-VSPbeta. The degradation pattern of S-VSPs in transgenic tobacco plants expressing both subunits resembled that of WT soybean. In contrast, the degradation pattern of transgenic tobacco plants expressing a single subunit was different. These finding suggest that the quaternary structure of S-VSPs may be an important factor determining their resistance to rumen degradation. Our results also suggest that the stability to rumen proteolysis of a given protein, when expressed in a transgenic plant, may not always be predictable and has to be verified.
机译：大豆营养贮藏蛋白（S-VSPs）富含赖氨酸，因此对于高产反刍动物具有很高的营养价值。使用来自野生型大豆和表达两个S-VSPs亚基之一（S-VSPalpha或S-VSbeta）之一或两者的转基因烟草植物的S-VSP，我们使用原位条件测试了它们在牛瘤胃液中的稳定性， SIDS-聚丙烯酰胺凝胶电泳。与野生型（WT）大豆植物相比，转基因烟草叶片中S-VSPs的蛋白水解和降解模式发生得相对较快。比较转基因植物中表达的两个S-VSPs亚基，我们发现S-VSPalpha的降解速度比S-VSPbeta快得多。在表达两个亚基的转基因烟草植物中，S-VSPs的降解模式与野生型大豆相似。相反，表达单个亚基的转基因烟草植物的降解模式是不同的。这些发现表明，S-VSP的四级结构可能是决定其抗瘤胃降解能力的重要因素。我们的结果还表明，当在转基因植物中表达时，给定蛋白质对瘤胃蛋白水解的稳定性可能并非总是可预测的，必须加以验证。

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