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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Liquid chromatography-mass spectrometry of cis- and all-trans-lycopene in human serum and prostate tissue after dietary supplementation with tomato sauce
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Liquid chromatography-mass spectrometry of cis- and all-trans-lycopene in human serum and prostate tissue after dietary supplementation with tomato sauce

机译：番茄酱膳食补充剂后人血清和前列腺组织中顺式和全反式番茄红素的液相色谱-质谱联用法

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Several epidemiological studies suggest a lower incidence of prostate cancer in men who routinely consume tomato products. Tomatoes are the primary dietary source of lycopene, which is among the most potent antioxidants of the carotenoids. Men with clinical stage T1 or T2 prostate adenocarcinoma were recruited (n = 32) and consumed tomato sauce based pasta dishes for 3 weeks (equivalent to 30 mg of lycopene per day) before radical prostectomy. Prostate tissue from needle biopsy just before intervention and prostectomy after supplementation from a subset of 11 subjects was evaluated for both total lycopene and lycopene geometrical isomer ratios. A gradient HPLC system using a C-18 column with UV-vis absorbance detection was used to measure total lycopene. Because the absorbance detector was insufficiently sensitive, HPLC with a C-30 column and positive ion atmospheric pressure chemical ionization mass spectrometric (LC-MS) detection was developed as a new assay to measure the ratio of lycopene cis/trans isomers in these samples. The limit of detection of the LC-MS method was determined to be 0.93 pmol of lycopene on-column, and a linear response was obtained over 3 orders of magnitude. Total lycopene in serum increased 2.0-fold from 35.6 to 69.9 mug/dL (from 0.664 to 1.30 muM) as a result of dietary supplementation with tomato sauce, whereas total lycopene in prostate tissue increased 3.0-fold from 0.196 to 0.582 ng/mg of tissue (from 0.365 to 1.09 pmol/mg). all-trans-Lycopene and at least 14 cis-isomer peaks were detected in prostate tissue and serum. The mean proportion of all-trans-lycopene in prostate tissue was similar to12.4% of total lycopene before supplementation but increased to 22.7% after dietary intervention with tomato sauce. In serum there was only a 2.8% but statistically significant increase in the proportion of all-trans-lycopene after intervention. These results indicate that short-term supplementation with tomato sauce containing primarily all-trans-lycopene (83% of total lycopene) results in substantial increases in total lycopene in serum and prostate and a substantial increase in all-trans-lycopene in prostate but relatively less in serum.
机译：几项流行病学研究表明，经常食用番茄制品的男性前列腺癌的发病率较低。番茄是番茄红素的主要饮食来源，番茄红素是类胡萝卜素中最有效的抗氧化剂之一。接受前列腺癌（T1或T2期临床分期）的男性（n = 32），并在根治性前列腺切除术前食用了以番茄酱为基础的意大利面食3周（相当于每天30毫克番茄红素）。评估了来自介入治疗前针刺活检的前列腺组织和补充了11位受试者的一部分后的前列腺切除术的总番茄红素和番茄红素几何异构体比率。使用带有UV-vis吸光度检测的C-18色谱柱的梯度HPLC系统测量总番茄红素。由于吸光度检测器不够灵敏，因此开发了带有C-30色谱柱的HPLC和正离子大气压化学电离质谱（LC-MS）检测作为测量这些样品中番茄红素顺式/反式异构体比例的新方法。 LC-MS方法的检出限确定为0.93 pmol番茄红素在柱上，并且在3个数量级上获得了线性响应。饮食中添加番茄酱的结果，血清中番茄红素的总含量从35.6杯/ dL（0.664至1.30μM）增加了2.0倍，而前列腺组织中番茄红素的总含量从0.196 ng / mg增加了3.0倍至0.582 ng / mg。组织（0.365至1.09 pmol / mg）。在前列腺组织和血清中检测到全反番茄红素和至少14个顺式异构体峰。补充前番茄组织中全反式番茄红素的平均比例约为总番茄红素的12.4％，但在番茄酱饮食干预后增加至22.7％。血清中全反式番茄红素的比例仅增加了2.8％，但具有统计学意义。这些结果表明，短期补充主要含全反式番茄红素（占番茄红素总量的83％）的番茄酱可导致血清和前列腺中总番茄红素的大量增加，而前列腺中全反番茄红素的大量增加，但相对而言血清中较少。

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