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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Rapid and sensitive determination of phenol in honey by high-performance liquid chromatography with fluorescence detection
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Rapid and sensitive determination of phenol in honey by high-performance liquid chromatography with fluorescence detection

机译：高效液相色谱-荧光检测法快速灵敏地测定蜂蜜中的苯酚

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摘要

The objective of this research was to develop a novel high-performance liquid chromatographic (HPLC) method involving a simple sample preparation procedure for the rapid, low-cost, and sensitive quantitation of phenol in honey at levels of regulatory and practical importance. After proper dilution of honey with water, the samples were analyzed by a gradient HPLC system, using a reversed-phase column with fluorescence detection at excitation and emission wavelengths of 270 and 300 nm, respectively. The eluents applied were water-acetonitrile-85% orthophosphoric acid (10:10: 0.01, v/v/v) and water-85% orthophosphoric acid (20:0.01, v/v). The retention time of phenol was found to be 14.1 min, and the limit of quantitation for phenol in honey was set at 5 mug/kg. Overall recovery was 98%. The proposed method has been successfully applied to real sample analysis.
机译：这项研究的目的是开发一种新颖的高效液相色谱（HPLC）方法，该方法涉及一种简单的样品制备程序，可对蜂蜜中的苯酚进行快速，低成本和灵敏的定量，具有监管和实际意义。蜂蜜用水适当稀释后，使用反相色谱柱通过梯度HPLC系统分析样品，该色谱柱分别在激发和发射波长分别为270和300 nm处进行荧光检测。施加的洗脱液为水-乙腈-85％正磷酸（10：10：0.01，v / v / v）和水-85％正磷酸（20：0.01，v / v）。苯酚的保留时间为14.1分钟，蜂蜜中苯酚的定量限为5杯/千克。总体恢复率为98％。所提出的方法已成功地应用于实际样品分析。

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