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首页> 外文期刊>Journal of Agricultural and Food Chemistry >SUPERCRITICAL FLUID EXTRACTION OF FUMONISIN B-1 FROM GRAIN DUST
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SUPERCRITICAL FLUID EXTRACTION OF FUMONISIN B-1 FROM GRAIN DUST

机译：从谷物粉中超临界萃取富马菌素B-1

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摘要

Fumonisin B-1 (FB1) was extracted from corn, corn dust, and Fusarium moniliforme culture samples using supercritical carbon dioxide (SC-CO2). The supercritical fluid extraction (SFE) conditions were optimized regarding the use of different SC-CO2 modifiers, modifier volume, pressure, temperature, and static extraction time. The addition of the modifier both in the extraction cell (prior to the static extraction step) and on-line with liquid carbon dioxide (during the dynamic SFE step) was found to significantly increase the recovery of FB1. Under the optimized SFE conditions (15 mL of liquid CO2, 750 mu L of 5% acetic acid per gram of sample, 1200 psi, 20 min static extraction time), the recovery of FB1 was found to be approximately 40 times greater than the recovery using conventional solvent extraction. SFE was faster and more reproducible (RSD = 3-5%) compared to the solvent extraction (RSD = 6.5%). The calculated detection limit was approximately 150 ppb, on the basis of the HPLC analysis of FB1-fluorescamine derivatives with UV detection.
机译：伏马菌素B-1（FB1）是使用超临界二氧化碳（SC-CO2）从玉米，玉米尘和镰刀形镰刀菌培养样品中提取的。针对不同的SC-CO2改性剂的使用，改性剂的体积，压力，温度和静态萃取时间，对超临界流体萃取（SFE）条件进行了优化。发现在萃取池中（在静态萃取步骤之前）和在线与液态二氧化碳（在动态SFE步骤期间）同时添加改性剂可显着提高FB1的回收率。在优化的SFE条件下（每克样品15 mL液态CO2、750μL5％乙酸，1200 psi，20分钟静态萃取时间），FB1的回收率约为回收率的40倍。使用常规溶剂萃取。与溶剂萃取（RSD = 6.5％）相比，SFE更快且重现性更高（RSD = 3-5％）。基于FB1-荧光胺衍生物的HPLC分析和UV检测，计算出的检出限约为150 ppb。

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