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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Activation of the Ah receptor by extracts of dietary herbal supplements, vegetables, and fruits
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Activation of the Ah receptor by extracts of dietary herbal supplements, vegetables, and fruits

机译：日粮草药补品，蔬菜和水果的提取物激活Ah受体

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The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor that can be activated by a structurally diverse range of synthetic and natural chemicals, and it mediates the toxic and biological effects of environmental contaminants such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The spectrum of chemicals that bind to and activate the AhR signal transduction pathway and the identity of materials containing AhR active chemicals is only now being defined. Utilizing AhR-dependent gel retardation and reporter gene bioassays, the screening of extracts of 22 dietary herbal supplements and 21 food products (vegetables and fruits) was performed to identify those containing AhR agonists. Several herbal extracts (ginseng, Fo-Ti, white oak bark, licorice, ginkgo biloba, and black cohosh) stimulated AhR DNA binding and gene expression to levels between 20 and 60% of that produced by TCDD. Although some food extracts (corn, jalapeno pepper, green bell pepper, apple, Brussels sprout, and potato) were relatively potent activators of AhR DNA binding (30-50% of TCDD), only corn and jalapeno pepper extracts induced AhR-dependent luciferase reporter gene expression. However, dilution of corn, jalapeno pepper, bell pepper, and potato extracts dramatically increased their ability to induce luciferase activity, suggesting that these extracts contained AhR antagonists whose effectiveness was overcome by dilution. Overall, these results demonstrate that dietary products can be a major source of naturally occurring AhR ligands to which animals and humans are chronically exposed.
机译：芳烃受体（AhR）是一种依赖配体的转录因子，可以被结构上多种多样的合成和天然化学物质激活，并介导环境污染物（例如2,3,7,8-四氯二苯并对二恶英（TCDD）。绑定和激活AhR信号转导途径的化学物质的光谱以及含有AhR活性化学物质的材料的身份现在才被确定。利用依赖于AhR的凝胶阻滞作用和报告基因生物测定法，对22种饮食草药补品和21种食品（蔬菜和水果）的提取物进行了筛选，以鉴定出含有AhR激动剂的提取物。几种草药提取物（人参，Fo-Ti，白橡树皮，甘草，银杏和黑升麻）刺激AhR DNA结合和基因表达达到TCDD产生的20％至60％的水平。尽管某些食物提取物（玉米，墨西哥胡椒，青椒，苹果，抱子甘蓝和马铃薯）是AhR DNA结合的相对有效激活剂（TCDD的30-50％），但只有玉米和墨西哥胡椒提取物诱导了AhR依赖的荧光素酶报告基因的表达。但是，稀释玉米，墨西哥胡椒，甜椒和马铃薯提取物会大大增强其诱导萤光素酶活性的能力，这表明这些提取物含有AhR拮抗剂，其有效性可通过稀释克服。总体而言，这些结果表明，饮食产品可能是动物和人类长期暴露于其中的天然存在的AhR配体的主要来源。

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