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首页> 外文期刊>Journal of Agricultural and Food Chemistry >GERMINATING RAPESEED AS BIOCATALYST - HYDROLYSIS OF OILS CONTAINING COMMON AND UNUSUAL FATTY ACIDS
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GERMINATING RAPESEED AS BIOCATALYST - HYDROLYSIS OF OILS CONTAINING COMMON AND UNUSUAL FATTY ACIDS

机译：作为生物催化剂的油菜种子的萌发-水解含常见和不常见脂肪酸的油

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摘要

Seedlings of low-erucic rape (Brassica napus) at day 3-5 of germination have been homogenized in Tris-HCl buffer and the homogenate used-as such or after defatting with pentane-as a biocatalyst for the hydrolysis of fats and oils containing common and unusual fatty acids. The rape seedlings were found to be highly active in the hydrolysis of triacylglycerols of linseed oil, castor oil, borage oil, coriander oil, Hydnocarpus wightiana oil, and hake (Merluccius hubbsi) liver oil but were unable to hydrolyze the wax esters contained in jojoba oil and orange roughy (Hoplostethus atlanticus) oil to any appreciable extent. In the hydrolysis of borage oil, coriander oil, H. wightiana oil, and hake liver oil the rape seedlings were found to discriminate against gamma-linolenoyl, petroselinoyl, gorlioyl and n-3 docosahexaenoyl moieties, respectively. These findings show the potentials of germinating rape seedlings as a cheap and easy-to-obtain biocatalyst for complete hydrolysis of oils or for their partial selective hydrolysis for the enrichment of definite fatty acids from mixtures.
机译：在Tris-HCl缓冲液中均质化低芥菜油菜（Brassica napus）幼苗，并在Tris-HCl缓冲液中均质化，然后使用均质液或戊烷脱脂后的均质液作为水解含常见油脂的生物催化剂。和不常见的脂肪酸研究发现，油菜幼苗在亚麻籽油，蓖麻油，琉璃苣油，香菜油，Hydnocarpus wightiana油和无须鳕（Merluccius hubbsi）肝油的三酰甘油水解中具有很高的活性，但无法水解荷荷巴油中所含的蜡酯。油和橙粗糙的（Hoplostethus atlanticus）油至任何明显的程度。在琉璃苣油，香菜油，H。wightiana油和无须鳕肝油的水解过程中，发现油菜幼苗分别区分γ-亚麻酸，petroselinoyl，gorlioyl和n-3二十二碳六烯酰基部分。这些发现表明，将油菜种子发芽作为廉价且易于获得的生物催化剂，有可能使油完全水解，或使其部分选择性水解，从而从混合物中富集确定的脂肪酸。

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