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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Quantification of cis-trans isomers of provitamin A carotenoids in fresh and processed fruits and vegetables.
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Quantification of cis-trans isomers of provitamin A carotenoids in fresh and processed fruits and vegetables.

机译：新鲜和加工的水果和蔬菜中维生素原A类胡萝卜素的顺反异构体的定量。

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A polymeric 5μm C30 stationary phase for reversed phase HPLC was used to separate and quantitate geometric isomers of provitamin A carotenoids in fresh and processed fruits and vegetables (broccoli, cantaloupe (Cucumis melo), carrots, kale, orangejuice, peaches, spinach, sweet potatoes, tomatoes and tomato-based vegetarian vegetable soup). beta-Carotene isomers (all-trans, 9-cis, 13-cis and 15-cis), alpha-carotene isomers (all-trans, 9-cis, 13-cis and 13'-cis), and beta-cryptoxanthin isomers (all-trans, 13 and 13'-cis and 15-cis) were resolved isocratically using the C30 stationary phase with 89:11 methanol/methyl tert-butyl ether as mobile phase. The percentage of cis isomers increased 10-39% with canning. The total provitamin A carotenoid content (鎔/g dry weight) ranged from 3.5 to 907 in fresh samples and from 1.8 to 1055 in canned samples. In several fruits and vegetables, processing produced an increase of 16-50% of total measured provitamin A carotenoids relative to the fresh samples. These increases were most likely a result of increased extraction efficiency, inactivation of enzymes capable of degrading carotenoids, and/or loss of soluble solids into the liquid canning medium.
机译：用于反相HPLC的聚合5μmC30固定相用于分离和定量原维生素A类胡萝卜素的几何异构体，包括新鲜和加工的水果和蔬菜（西兰花，哈密瓜（Cucumis melo），胡萝卜，羽衣甘蓝，橙汁，桃子，菠菜，甘薯） ，西红柿和以西红柿为基础的素食蔬菜汤）。 β-胡萝卜素异构体（全反式，9-顺式，13-顺式和15-顺式），α-胡萝卜素异构体（全反式，9-顺式，13-顺式和13'-顺式）和β-隐黄质异构体使用C30固定相，以89:11甲醇/甲基叔丁基醚作为流动相，等度拆分（全反式，13和13'-顺式和15-顺式）。装罐后，顺式异构体的百分比增加了10-39％。新鲜样品中维生素原A的总类胡萝卜素含量（镕/ g干重）为3.5至907，罐装样品中为1.8至1055。相对于新鲜样品，在几种水果和蔬菜中，加工产生的总维生素原A类胡萝卜素含量增加了16-50％。这些增加很可能是提取效率提高，能够降解类胡萝卜素的酶失活和/或液体罐装介质中可溶性固体损失的结果。

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