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首页> 外文期刊>Journal of Agricultural and Food Chemistry >DISPOSITION OF ORAL [C-14]SULFATHIAZOLE IN SWINE
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DISPOSITION OF ORAL [C-14]SULFATHIAZOLE IN SWINE

机译：口服[C-14]磺胺唑在猪中的分布

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Disposition of oral sulfathiazole was studied in swine. Pigs were slaughtered 6, 12, 24, and 48 h after an oral dose of [C-14]sulfathiazole (two at each time period). Excretion of C-14 was rapid (>90% in 48 h), primarily via the urine. Metabolites isolated and characterized by H-1 NMR and FAB MS were N-4-acetylsulfathiazole from urine, kidney, liver, blood, and muscle; N-4-glucoside of sulfathiazole from muscle; and an apparent diconjugate from liver, a glucuronide of N-4-acetylsulfathiazole. Quantitation was accomplished by HPLC analysis of samples (extracts of tissue and urine) spiked with the reference compounds. Peaks corresponding to the retention time of the reference compounds were trapped and assayed for C-14. Sulfathiazole and N-4-acetylsulfathiazole were the principal C-14-labeled compounds in urine and kidney. If present, the glucoside or glucuronide represented <5% of the C-14 in urine. Liver and muscle contained significant amounts of sulfathiazole, N-4-acetylsulfathiazole, and N-4-glucoside of sulfathiazole. Quantitation of the diconjugate was not attempted. The amounts isolated suggest it was a minor metabolite; however, instability during isolation was apparent. On the basis of the results, liver, kidney, and urine are potential target tissues for a residue monitoring program and the nature of the metabolite present in the tissue must be considered when an assay procedure is selected.
机译：在猪中研究了口服磺胺噻唑的处置。口服[C-14]磺胺噻唑后6小时，12小时，24小时和48小时宰杀猪（每次2头）。 C-14的排泄迅速（在48小时内> 90％），主要是通过尿液排泄。通过H-1 NMR和FAB MS分离并表征的代谢物是来自尿液，肾脏，肝脏，血液和肌肉的N-4-乙酰基磺胺噻唑;肌肉中磺胺噻唑的N-4-葡萄糖苷;和一个明显的肝脏双缀合物，N-4-乙酰基磺胺噻唑的葡糖醛酸。通过HPLC分析加标有参考化合物的样品（组织和尿液的提取物）来完成定量。捕获对应于参考化合物保留时间的峰，并分析其C-14。磺胺噻唑和N-4-乙酰基磺胺噻唑是尿液和肾脏中主要的C-14标记化合物。如果存在，则葡萄糖苷或葡糖醛酸苷占尿液中C-14的<5％。肝脏和肌肉中含有大量的硫代噻唑，N-4-乙酰基硫代噻唑和N-4-葡萄糖苷。未尝试定量二轭合物。分离出的数量表明它是次要的代谢产物。但是，隔离期间的不稳定性很明显。根据结果​​，肝，肾和尿液可能是残留监测程序的潜在目标组织，并且在选择测定程序时必须考虑组织中存在的代谢物的性质。

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