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首页> 外文期刊>Journal of Agricultural and Food Chemistry >BINDING OF POLY(ETHYLENE GLYCOL) TO SAMPLES OF FORAGE PLANTS AS AN ASSAY OF TANNINS AND THEIR NEGATIVE EFFECTS ON RUMINALL DEGRADATION
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BINDING OF POLY(ETHYLENE GLYCOL) TO SAMPLES OF FORAGE PLANTS AS AN ASSAY OF TANNINS AND THEIR NEGATIVE EFFECTS ON RUMINALL DEGRADATION

机译：聚乙烯醇与饲草植物样品的单宁结合及其对瘤胃降解的消极作用

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摘要

A procedure to measure poly(ethylene glycol) (PEG) binding to plant samples is described. The amount of PEG bound to a sample was found to be reproducible with a small coefficient of variation (0.5%). The PEG-binding capacity of plant samples was not affected by predrying of the samples at 90 degrees C, unlike the colorimetric methods for determination of tannins. Both the PEG-binding and the protein precipitation capacity of the plant samples were found to be useful in predicting the negative effects of tannins on ruminal degradation of the plant material. However, PEG binding to plant samples had an advantage over protein precipitation in samples in which formation of strong tannin-protein complexes results in low extractability of tannins. For this reason, the binding method was found to be preferable to the protein precipitation method in predicting the negative effect of tannins on ruminal degradation of forage plants.
机译：描述了测量聚（乙二醇）（PEG）与植物样品结合的方法。发现与样品结合的PEG量可重现，变异系数很小（0.5％）。不同于比色法测定单宁的方法，植物样品的PEG结合能力不受样品在90摄氏度下的预干燥的影响。已发现植物样品的PEG结合能力和蛋白质沉淀能力都可用于预测单宁酸对植物材料瘤胃降解的负面影响。但是，PEG与植物样品的结合优于样品中的蛋白质沉淀，在样品中形成强力单宁-蛋白质复合物会导致单宁的低提取性。因此，在预测单宁酸对饲用植物瘤胃降解的负面影响时，发现结合方法优于蛋白质沉淀方法。

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