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Optimization of Extraction of Fpase from the Fermented Bran of Aspergillus niger in Solid State Fermentation

机译:固态发酵黑曲霉发酵麸皮中Fpase的提取工艺优化

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摘要

A local isolate of Aspergillus niger was cultivated under optimal growth conditions on wheat bran in solid state fermentation. Filter paperase from fermented bran was separately extracted with different solvents to test the recovery of the enzyme. Among solvents tested, distilled water served as the best leachate, thus the conditions were further optimized with distilled water. After two washes of fermented bran with distilled water for 1.5 h each under stationary conditions at 1 g wheat bran : 5 mLdistilled water, the maximum recovery of 13.5 U g~(-1) of wheat bran was obtained.
机译:在最佳发酵条件下,在固态发酵的麦麸上培养了黑曲霉的局部分离株。用不同的溶剂分别提取发酵麸皮中的滤纸酶,以测试酶的回收率。在测试的溶剂中,蒸馏水是最好的浸出液,因此使用蒸馏水可以进一步优化条件。在固定条件下,于1 g麦麸:5 mL蒸馏水中,用蒸馏水将发酵过的麸皮洗涤两次,每次1.5 h,得到的最大回收量为13.5 U g〜(-1)。

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