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首页> 外文期刊>Journal of AOAC International >Simultaneous determination of free capsorubin and capsanthin in red pepper powder using u-HPLC.
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Simultaneous determination of free capsorubin and capsanthin in red pepper powder using u-HPLC.

机译：使用u-HPLC同时测定红辣椒粉中的游离辣椒红素和辣椒红素。

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摘要

A simultaneous ultra-HPLC (u-HPLC) method for determination of free capsorubin and capsanthin in red pepper powder was validated in terms of its precision, accuracy and linearity. The u-HPLC separation was performed on an RP C18 column (particle size 2 mum, id 2 mm length 100 mm) and with a photodiode-array detector. Recoveries of capsorubin were >83.8 +or- 1.7%, and LOD and LOQ of the u-HPLC analyses were 0.043 and 0.129 mg/kg, respectively. Intraday and interday precisions for capsorubin were <9.01%. Recoveries of capsanthin were >87.7 +or- 1.5%, and LOD and LOQ were 0.101 and 0.306 mg/kg, respectively. Intraday and interday precisions for capsanthin were <12.66%. All calibration curves for capsorubin and capsanthin exhibited good linearity (r2 = 0.99) within the tested ranges.
机译：通过同时的超高效液相色谱法（u-HPLC）测定了红辣椒粉中游离的辣椒红素和辣椒红素的准确性，准确性和线性度。 u-HPLC分离是在RP C18色谱柱（粒径2毫米，内径2毫米，长度100毫米）上和光电二极管阵列检测器上进行的。辣椒红素的回收率> 83.8 +或-1.7％，u-HPLC分析的LOD和LOQ分别为0.043和0.129 mg / kg。辣椒红素的日内和日间精确度均小于9.01％。辣椒红素的回收率> 87.7±1.5％，LOD和LOQ分别为0.101和0.306 mg / kg。辣椒红素的日内和日间精确度均<12.66％。在测试范围内，辣椒红素和辣椒红素的所有校准曲线均显示出良好的线性（r 2 = 0.99）。

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