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首页> 外文期刊>Journal of Apicultural Research >Oxytetracycline-resistance in the honey bee pathogen Paenibacillus larvae is encoded on novel plasmid pMA67.
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Oxytetracycline-resistance in the honey bee pathogen Paenibacillus larvae is encoded on novel plasmid pMA67.

机译：蜜蜂病原体幼芽孢杆菌幼虫中的抗土霉素抗性在新型质粒pMA67上编码。

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摘要

The gram-positive bacterium, Paenibacillus larvae, causes a serious honey bee disease, American Foulbrood. For several decades, commercial and hobbyist beekeepers have controlled this disease with the antibiotic oxytetracycline. However, in recent years there have been reports of oxytetracycline-resistant P. larvae. In this study, we report that the reason for the oxytetracycline resistance in P. larvae is the presence of a novel plasmid carrying a tetracycline resistance gene - tetL. We tested 36 strains of P. larvae from the USA and Canada and found this plasmid in all 21 oxytetracycline-resistant strains and in none of the 15 oxytetracycline-sensitive strains. We cloned and expressed the P. larvae tetL gene in Escherichia coli and showed it was functional. Sequencing of the entire plasmid, which we named pMA67, revealed that it is likely a mobilizable rolling-circle replication plasmid. This work provides the first sequence information for any P. larvae plasmid, a new tetL ortholog with significant sequence divergence from tetL genes found in other species, and the first tetracycline-resistance gene found in the entire Paenibacillus genus..
机译：革兰氏阳性细菌幼虫Paenibacillus会引起严重的蜜蜂疾病，美国Foulbrood。几十年来，商业和业余养蜂人使用抗生素土霉素来控制这种疾病。但是，近年来有耐土霉素的幼虫的报道。在这项研究中，我们报告在幼虫中对土霉素抗性的原因是存在一个带有四环素抗性基因-tetL的新型质粒。我们测试了来自美国和加拿大的36株幼虫疟原虫菌株，并在所有21株对土霉素抗性菌株中发现了该质粒，而在15株对土霉素敏感的菌株中均未发现该质粒。我们在大肠杆菌中克隆并表达了幼虫tetL基因，并证明其具有功能。整个质粒（我们命名为pMA67）的测序表明，它很可能是一种可移动的滚环复制质粒。这项工作提供了任何P.幼虫质粒的第一个序列信息，一个新的tetL直向同源物，其与其他物种的tetL基因有明显的序列差异，以及第一个四环素抗性基因在整个杆状芽孢杆菌属中发现。

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