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首页> 外文期刊>Journal of Applied Polymer Science >A novel approach for albumin determination in aqueous media by using temperature- and pH-sensitive N-isopropylacrylamide-co-N-[3-(dimethylamino)-propyl]methacrylamide random copolymers
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A novel approach for albumin determination in aqueous media by using temperature- and pH-sensitive N-isopropylacrylamide-co-N-[3-(dimethylamino)-propyl]methacrylamide random copolymers

机译：使用温度和pH敏感的N-异丙基丙烯酰胺-co-N- [3-（二甲氨基）-丙基]甲基丙烯酰胺无规共聚物测定水性介质中白蛋白的新方法

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Random copolymers of N-isopropylacrylamide (NIPA) and N-[3-(dimethylamino)propyl]methacrylamide (DMAPM) were synthesized by solution polymerization using azobisizobutyronitrile as the initiator in 1,4-dioxane at. 60 degreesC. NIPA-co-DMAPM copolymer exhibited both temperature and pH sensitivity. Thermally reversible phase transitions were observed both in the acidic and the alkaline pH regions for copolymers produced with different DMAPM/NIPA feed ratios. The pH dependency of the lower critical solution temperature (LCST) was stronger for copolymers produced with higher DMAPM feed concentrations. NIPA-co-DMAPM random copolymer was also sensitive to the albumin concentration. In the presence of albumin, thermally irreversible phase transitions were observed in slightly acidic and neutral media. However, reversible transitions were obtained in aqueous media containing albumin-at basic pH. The phase-transition temperature of NIPA-co-DMAPM copolymer significantly decreased with increasing albumin concentration at both acidic and alkaline pH values. This behavior was explained by albumin binding onto the copolymer chains by means of H-bond formation between the dimethylamino groups of the copolymer and the carboxyl groups of albumin. For a certain range of albumin concentration, the phase-transition temperature exhibited a linear decrease with increasing albumin concentration. By utilizing this behavior, a simple albumin assay was developed. The results indicated that NIPA-co-DMAPM copolymer could he utilized as a new reagent for the determination of albumin concentration in the aqueous medium. The proposed method was valid for the albumin concentration range of 0-4000 mug/mL. The protein concentrations commonly utilized in biotechnological studies fall in the range of the proposed method. (C) 2002 Wiley Pehodicals, Inc. [References: 45]
机译：N-异丙基丙烯酰胺（NIPA）和N- [3-（二甲基氨基）丙基]甲基丙烯酰胺（DMAPM）的无规共聚物是通过偶氮二偶氮丁腈在1,4-二恶烷中作为引发剂进行溶液聚合而合成的。 60℃。 NIPA-co-DMAPM共聚物同时具有温度和pH敏感性。对于使用不同DMAPM / NIPA进料比生产的共聚物，在酸性和碱性pH区域均观察到热可逆相变。对于具有较高DMAPM进料浓度的共聚物，较低的临界溶液温度（LCST）对pH的依赖性较强。 NIPA-co-DMAPM无规共聚物对白蛋白浓度也敏感。在白蛋白存在下，在弱酸性和中性介质中观察到热不可逆的相变。然而，在碱性条件下，在含有白蛋白的水性介质中获得了可逆的转变。 NIPA-co-DMAPM共聚物的相变温度随着在酸性和碱性pH值下白蛋白浓度的增加而显着降低。通过在共聚物的二甲基氨基和白蛋白的羧基之间通过H键形成将白蛋白结合到共聚物链上来解释这种行为。对于一定范围的白蛋白浓度，相变温度随白蛋白浓度的增加呈线性下降。通过利用这种行为，开发了一种简单的白蛋白测定法。结果表明，NIPA-co-DMAPM共聚物可作为测定水介质中白蛋白浓度的新试剂。所提出的方法对白蛋白浓度范围为0-4000杯/毫升有效。在生物技术研究中通常使用的蛋白质浓度在提出的方法范围内。 （C）2002 Wiley Pehodicals，Inc. [参考：45]

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