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首页> 外文期刊>Journal of Applied Polymer Science >Immobilization of glucose oxidase and acetylcholinesterase onto modified polyamide sorbent
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Immobilization of glucose oxidase and acetylcholinesterase onto modified polyamide sorbent

机译：将葡萄糖氧化酶和乙酰​​胆碱酯酶固定在改性聚酰胺吸附剂上

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Two types of polyamide (PA) sorbents with high specific area were prepared. The effects of solvent type, concentrations of formic acid, and polymer on the porosity characteristics were studied. The sorbent with the highest specific area was obtained by using C2H5OH-HCOOH solvent (60% HCOOH) and the rest of the experiments were carried out with this type of sorbent. The possibility of applying the PA sorbent as carrier for immobilization of glucose oxidase (GOD) and acetylcholinesterase (AChE) was investigated. In order to increase the active groups content (necessary for enzyme immobilization), the sorbent was modified with dimethylaminoethylmethacrylate (DMAEM) and 2-acrylamido-2-methylpropensulfonic acid. The amount of the active groups introduced during the modification and the degree of hydrophilicity were determined. The quantity of bound protein and relative activity of GOD and AChE immobilized onto unmodified and modified sorbents were studied. Optimum pH and temperature of the immobilized GOD and AChE were also determined. The influence of three phosphoroorganic compounds on the activity of the immobilized AChE was investigated. Tetrachlorvinvos was found to be the strongest inhibitor, while AChE immobilized onto PA sorbent modified with DMAEM showed the highest stability. The possibility of using immobilized GOD and AChE in a flow-injection system for determination of the concentrations of glucose and phosphoroorganic compounds was studied. (C) 1998 John Wiley & Sons, Inc. [References: 22]
机译：制备了两种具有高比表面积的聚酰胺（PA）吸附剂。研究了溶剂类型，甲酸浓度和聚合物对孔隙率特性的影响。通过使用C2H5OH-HCOOH溶剂（60％HCOOH）获得比表面积最高的吸附剂，其余的实验均使用这种类型的吸附剂进行。研究了将PA吸附剂作为载体固定化葡萄糖氧化酶（GOD）和乙酰胆碱酯酶（AChE）的可能性。为了增加活性基团含量（酶固定所必需的），用甲基丙烯酸二甲基氨基乙基酯（DMAEM）和2-丙烯酰胺基-2-甲基丙磺酸对吸附剂进行了改性。确定了改性期间引入的活性基团的量和亲水性的程度。研究了固定在未改性和改性吸附剂上的结合蛋白的量以及GOD和AChE的相对活性。还确定了固定的GOD和AChE的最佳pH和温度。研究了三种磷酸有机化合物对固定化AChE活性的影响。发现四氯乙烯是最强的抑制剂，而固定在DMAEM改性的PA吸附剂上的AChE表现出最高的稳定性。研究了在流动注射系统中使用固定化的GOD和AChE测定葡萄糖和磷酸有机化合物浓度的可能性。 （C）1998 John Wiley＆Sons，Inc. [参考：22]

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