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首页> 外文期刊>Journal of Applied Polymer Science >Influence of laboratory ware related changes in conformational and mechanical properties of collagen
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Influence of laboratory ware related changes in conformational and mechanical properties of collagen

机译：影响实验室用品的胶原蛋白构象和机械性能变化

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摘要

Enzyme-treated acid-solubilized bovine collagen (ESBC Type I) prepared in laboratory-grade glass and polypropylene ware under identical conditions have shown different conformational and mechanical properties. Circular dichroism (CD) studies on ESBC have shown higher triple-helical contents in polypropylene laboratory-grade ware compared to laboratory ware of glass. Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetric (DSC) studies have supported the conformational changes performed on both preparations. Both preparations, in membrane form, were evaluated for their mechanical properties-viz., shrinkage temperature (St), dissolution temperature (Dt), and tensile strength (Ts). The collagen membrane of ESBC prepared in polypropylene showed significantly higher values compared to glass ones. No difference was seen in shrinkage temperature data of the two membranes. Reduced viscosity of ESBC was also measured for both preparations, and it had a significantly higher value in polypropylene ware compared to ESBC prepared in glassware. We postulate that the presence of silicate ions (SiO3-2) in glassware has a role in all these changes. (C) 2003 Wiley Periofficals, Inc. [References: 35]
机译：在相同条件下在实验室级玻璃和聚丙烯器皿中制备的经酶处理的酸增溶牛胶原蛋白（ESBC I型）显示出不同的构象和机械性能。在ESBC上进行的圆二色性（CD）研究表明，与玻璃实验室器皿相比，聚丙烯实验室级器皿中的三螺旋含量更高。傅里叶变换红外光谱（FTIR）和差示扫描量热法（DSC）研究已经支持了两种制剂的构象变化。评估了两种膜形式的制剂的机械性能，即收缩温度（St），溶解温度（Dt）和拉伸强度（Ts）。与玻璃相比，用聚丙烯制备的ESBC胶原膜的值明显更高。两种膜的收缩温度数据均未见差异。还测量了两种制剂的ESBC粘度降低，与在玻璃器皿中制备的ESBC相比，其在聚丙烯器皿中具有明显更高的价值。我们假设玻璃器皿中硅酸盐离子（SiO3-2）的存在与所有这些变化有关。 （C）2003 Wiley Periofficals，Inc. [参考：35]

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