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ENZYME AND CHEMICAL ENCAPSULATION IN POLYMERIC MICROCAPSULES

机译:聚合物微胶囊中的酶和化学包封

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Polypyrrole microcapsules (prepared via the template method) were used for immobilization of both enzymatic and chemical catalytic systems. Enzymes immobilized include glucose oxidase, catalase, trypsin, subtilisin, and alcohol dehydrogenase. The chemical catalytic system investigated consisted of immobilized Pd nanoparticles for the catalysis of hydrogen peroxide decomposition. Microcapsules loaded with glucose oxidase (GOD) were found to have higher enzymatic activity than that of GOD-loaded thin films, a competing encapsulation method. Trypsin was used to explore the possible leakage of small proteins from the capsules; no leakage was observed. Subtilisin was used to show that these microcapsules can be used in nonaqueous solvents. The effect of the capsule wall thickness on the rate of the enzymatic reaction was also explored. (C) 1996 John Wiley & Sons, Inc. [References: 39]
机译:聚吡咯微胶囊(通过模板法制备)用于固定酶和化学催化系统。固定的酶包括葡萄糖氧化酶,过氧化氢酶,胰蛋白酶,枯草杆菌蛋白酶和乙醇脱氢酶。研究的化学催化体系由固定化的Pd纳米颗粒组成,用于催化过氧化氢分解。发现与葡萄糖包裹的薄膜相比,葡萄糖氧化酶(GOD)的微胶囊具有更高的酶活性,这是一种竞争性的封装方法。胰蛋白酶用于探索胶囊中小蛋白的可能泄漏;没有观察到泄漏。枯草杆菌蛋白酶被用来表明这些微胶囊可以在非水溶剂中使用。还探讨了胶囊壁厚对酶促反应速率的影响。 (C)1996 John Wiley&Sons,Inc. [参考:39]

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