• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Applied Polymer Science >β-Galactosidase immobilization into poly(hydroxyethyl methacrylate) membrane and performance in a continuous system
【24h】

β-Galactosidase immobilization into poly(hydroxyethyl methacrylate) membrane and performance in a continuous system

机译：β-半乳糖苷酶固定在聚甲基丙烯酸羟乙酯膜中的性能及连续系统的性能

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The activity of β-galactosidase immobilized into a poly(2-hydroxyethyl methacrylate) (pHEMA) membrane increased from 1.5 to 10.8 U/g pHEMA upon increase in enzyme loading. The Km values for the free and the entrapped enzyme were found to be 0.26 and 0.81 mM, respectively. The optimum reaction temperatures for the free and the entrapped β-galactosidase were both found to be 50 °C. Similarly, the optimum reaction pH was 7.5 for both the free and the entrapped enzyme. The immobilized β-galactosidase was characterized in a continuous system during lactose hydrolysis and the operational inactivation rate constant (kiop) of the entrapped enzyme was found to be 3.1×10-5 min-1.
机译：固定在聚甲基丙烯酸2-羟乙酯（pHEMA）膜上的β-半乳糖苷酶的活性随酶负荷的增加从1.5增加到10.8 U / g pHEMA。发现游离酶和捕获的酶的Km值分别为0.26和0.81mM。发现游离的和截留的β-半乳糖苷酶的最佳反应温度均为50℃。类似地，游离酶和截留的酶的最佳反应pH均为7.5。固定化的β-半乳糖苷酶在乳糖水解过程中的连续系统中得到表征，并且所捕获的酶的操作失活速率常数（koop）为3.1×10-5 min-1。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号