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Preparation and characterization of poly(dimethyldiallyl ammonium) chloride and antiglobulin tests for antibody detection

机译:聚(二甲基二烯丙基铵)氯化物的制备,表征和抗球蛋白测试,用于抗体检测

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Poly(dimethyldiallyl ammonium)chloride [poly(DMDAAC)] was prepared in our laboratory and monomer-to-polymer structures were characterized by infrared (IR), proton, and carbon-13 nuclear magnetic resonance (NMR) as well as DEPT techniques. The polyelectrolytes were used to detect the antibodies of red blood cells (RBCs) in human sera and the results were compared with hexadimethrine bromide (polybrene), bromeline, and other methods. This article presents data supporting the use of the manual poly(DMDAAC) test; the 1-min procedure at room temperature provided a rapid and sensitive test. The states of agglutination of RBCs were investigated by transmission electron microscopy (TEM). The results of the morphology were in agreement with the manual poly(DMDAAC) test. (C) 2003 Wiley Periodicals, Inc. [References: 8]
机译:在我们的实验室中制备了聚二甲基二烯丙基氯化铵[poly(DMDAAC)],并通过红外(IR),质子和碳13核磁共振(NMR)以及DEPT技术对单体-聚合物结构进行了表征。聚电解质用于检测人血清中的红细胞(RBC)抗体,并将结果与​​六溴二苯醚溴化物(polybrene),溴甲烷和其他方法进行比较。本文介绍了支持使用人工poly(DMDAAC)测试的数据;室温下1分钟的程序提供了快速而灵敏的测试。通过透射电子显微镜(TEM)研究了RBC的凝集状态。形态学结果与人工聚(DMDAAC)测试一致。 (C)2003 Wiley Periodicals,Inc. [参考:8]

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