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首页> 外文期刊>Journal of Aquatic Sciences >THE COMPARATIVE HISTOLOGY OF THE TESTIS OF TWO CULTURED CLARIID SPECIES (Clarias gariepinus and Heterobranchus bidorsalis)
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THE COMPARATIVE HISTOLOGY OF THE TESTIS OF TWO CULTURED CLARIID SPECIES (Clarias gariepinus and Heterobranchus bidorsalis)

机译：两种养殖克拉丽科动物的睾丸的比较组织学（Clarias gariepinus和Heterobranchus bidorsalis）

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摘要

Sexually active (brood stock) males of Clarias gariepinus and Heterobranchus bidorsalis were histologically examined in this experiment. The objective is to examine histologically the testes of these two species and to compare their testicular structures with one another. The specimens were dissected and their testes removed whole and preserved in Bouin's fluid for 72hours. Tissue samples were collected and dehydrated through graded concentrations of alcohol. The tissues were cleared in two jars of xylene for one hour and then embedded after passing through the.four changes of paraffin wax at 60°C. Paraffin sections of 5micron thick were mounted on clear albuminized slides, dried in an oven and stained with Haematoxylin and Eosin (H & E) and Periodic Acid Schiff (PAS) and subsequently viewed under light microscope. Our findings showed that the seminiferous tubules of the Clarias gariepinus testis had wider lumen than those found in Heterobranchus bidorsalis. On the other hand the interlobular spaces were extensive in the Heterobranchus bidorsalis than that of Clarias gariepinus. The findings can serve as foundation to further research on the structural and ultrastructural variations in the testes of these species thereby enhancing the breeding and the hybridization of both species for genetic improvement.
机译：在该实验中，对Clarias gariepinus和Heterobranchus bidorsalis的有性活动的雄性（亲鱼）进行了组织学检查。目的是从组织学上检查这两个物种的睾丸，并相互比较它们的睾丸结构。解剖标本，将睾丸全部切除，并在布恩氏液中保存72小时。收集组织样品，并通过分级浓度的酒精进行脱水。在两个二甲苯罐中将组织清除一小时，然后在60°C通过四次石蜡后包埋。将5微米厚的石蜡切片安装在透明的，已被白蛋白化的载玻片上，在烤箱中干燥，并用苏木精和曙红（H＆E）和高碘酸希夫（PAS）染色，随后在光学显微镜下观察。我们的研究结果表明，Clarias gariepinus睾丸的曲细精管的内腔比在Heterobranchus bidorsalis中发现的管腔宽。另一方面，在异叶杂种中的小叶间空间比对虾（Clarias gariepinus）的小。这些发现可作为进一步研究这些物种的睾丸结构和超微结构变异的基础，从而增强这两个物种的繁殖和杂交，从而改善遗传。

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