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首页> 外文期刊>Journal of applied toxicology >Rat liver clone-9 cells in culture as a model for screening hepatotoxic potential of food-related products: hepatotoxicity of deoxynivalenol.
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Rat liver clone-9 cells in culture as a model for screening hepatotoxic potential of food-related products: hepatotoxicity of deoxynivalenol.

机译：培养的大鼠肝克隆9细胞可作为筛选食品相关产品的肝毒性潜力的模型：脱氧雪茄烯醇的肝毒性。

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Deoxynivalenol (DON) is a mycotoxin food contaminant found in several cereal grains. The literature on the liver toxicity of DON in vivo is conflicting and does not clearly characterize its hepatotoxic effects. Cultured rat liver clone-9 cells were used as a model to assess the hepatotoxic potential of DON. The cell cultures, seeded onto 96-well plates, were treated at confluence with varying concentrations of DON (0-100 microg ml(-1)) for 48 h at 37 degrees C in 5% CO2. After the treatment period, the cells were assayed for a number of hepatotoxic endpoints that included cytotoxicity, double-stranded DNA (ds-DNA) content, oxidative stress and mitochondrial function. The concentration-dependent toxicity of DON, as measured by cytotoxicity and ds-DNA content, was observed over the entire concentration range studied beginning at 0.5 microg ml(-1). DON also induced a significant concentration-dependent increase in oxidative stress at DON concentrations starting at 10 microg ml(-1). The mitochondrial function of the treated cells decreased with the increasing concentration of DON exposure, but it was not statistically different from that of the control value. Liver histopathology observed at 3, 24 and 72 h following a single intraperitoneal administration dose of DON (10 mg kg(-1) BW) to adult male rats is consistent with early mild hepatotoxicity. The overall results of this study suggest that acute DON exposure has early mild cytotoxic effects on hepatocytes in vivo that are expressed as severe effects in rat liver clone-9 cells in vitro.
机译：脱氧雪腐烯醇（DON）是一种真菌毒素食品污染物，存在于几种谷物中。关于DON在体内的肝毒性的文献相互矛盾，并且没有清楚地表征其肝毒性作用。培养的大鼠肝克隆9细胞用作评估DON的肝毒性潜力的模型。将细胞培养物接种到96孔板上，在37°C和5％CO2中用不同浓度的DON（0-100 microg ml（-1））融合处理48小时。在治疗期之后，分析细胞的许多肝毒性终点，包括细胞毒性，双链DNA（ds-DNA）含量，氧化应激和线粒体功能。通过细胞毒性和ds-DNA含量测量，DON的浓度依赖性毒性在研究的整个浓度范围（从0.5 microg ml（-1）开始）中观察到。 DON在DON浓度从10 microg ml（-1）开始也引起氧化应激的显着浓度依赖性增加。处理的细胞的线粒体功能随DON暴露浓度的增加而降低，但与对照组的值无统计学差异。对成年雄性大鼠单次腹膜内给予DON（10 mg kg（-1）BW）后3、24和72小时观察到的肝脏组织病理学与早期轻度肝毒性一致。这项研究的总体结果表明，急性DON暴露对体内的肝细胞具有早期的轻度细胞毒性作用，在体外大鼠肝克隆9细胞中表现为严重的作用。

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