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首页> 外文期刊>Journal of applied toxicology >Protective effect of C(60) -methionine derivate on lead-exposed human SH-SY5Y neuroblastoma cells.
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Protective effect of C(60) -methionine derivate on lead-exposed human SH-SY5Y neuroblastoma cells.

机译：C（60）-蛋氨酸衍生物对铅暴露的人SH-SY5Y神经母细胞瘤细胞的保护作用。

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Oxidative stress has been considered as one of the possible mechanisms leading to the neurotoxicity of lead. One of the effective ways to prevent cellular damage after lead exposure is using antioxidants. In this paper, a novel C(60) -methionine derivate (FMD), a fullerene molecule modified with methionine, was synthesized. The protective effect of FMD on lead-exposed human SH-SY5Y neuroblastoma cells was investigated. In this research, after incubating with 500 microm Pb acetate alone for 72 h, the cells had undergone a series of biological changes including viability loss, apoptotic death, the depletion of glutathione (GSH), the peroxidation of membrane lipid and DNA damage. Pretreatment with FMD before lead exposure could improve cell survival, increase the GSH level, reduce malondialdehyde content and attenuate DNA damage without obvious toxicity. In addition, the protective effects of FMD were proven to be greater than those of other two C(60) -amino acid derivates, beta-alanine C(60) derivate and cystine C(60) derivate, which have been confirmed in our previous work to be able to protect rat pheochromocytoma PC12 cells from hydrogen dioxide-induced oxidative injuries. These observations suggest that FMD may serve as a potential antioxidative and neuroprotective agent in the prevention of lead intoxication.
机译：氧化应激被认为是导致铅神经毒性的可能机制之一。防止铅暴露后细胞受损的有效方法之一是使用抗氧化剂。在本文中，合成了一种新型的C（60）-蛋氨酸衍生物（FMD），一种用蛋氨酸修饰的富勒烯分子。研究了FMD对铅暴露的人SH-SY5Y神经母细胞瘤细胞的保护作用。在这项研究中，仅与500微米醋酸铅一起孵育72小时后，细胞发生了一系列生物学变化，包括活力丧失，凋亡死亡，谷胱甘肽（GSH）消耗，膜脂质过氧化和DNA损伤。在暴露于铅之前用FMD进行预处理可以提高细胞存活率，提高GSH水平，降低丙二醛含量并减轻DNA损伤，而没有明显的毒性。此外，FMD的保护作用被证明比其他两个C（60）-氨基酸衍生物，β-丙氨酸C（60）衍生物和胱氨酸C（60）衍生物的保护作用更大，这在我们之前的研究中得到了证实。能够保护大鼠嗜铬细胞瘤PC12细胞免受二氧化氢诱导的氧化损伤的影响。这些观察结果表明，FMD可以在预防铅中毒方面充当潜在的抗氧化剂和神经保护剂。

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