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首页> 外文期刊>Journal of applied toxicology >Short-term splenic impact of single-strand DNA functionalized multi-walled carbon nanotubes intraperitoneally injected in rats
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Short-term splenic impact of single-strand DNA functionalized multi-walled carbon nanotubes intraperitoneally injected in rats

机译：大鼠腹膜内注射单链DNA功能化的多壁碳纳米管的短期脾脏影响

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摘要

In recent years, a great deal of studies have focused on the possible toxicity of carbon nanotubes (CNT), as a result of their potential applications in the field of nanotechnologies. The investigation of spleen toxicity is part of the carbon nanotubes-induced toxicity assessment. In this study, we investigated the possible toxic effects of CNT on the rat spleen, after intraperitoneally (i.p.) administration of a single dose [1.5 ml; 2 mg multi-walled (MW) CNT per body weight (bw)] of multi-walled carbon nanotubes (exterior diameter 15-25 nm, interior diameter 10-15 nm, surface 88 m2 g-1) functionalized 1:1 with single-strand DNA (ss-DNA-MWCNT, 270 mg l-1). CNT functionalization with DNA determines a stable dispersion in the body fluids. For the detection of carbon nanotubes in the spleen, Raman spectroscopy, histopathologic examination, confocal microscopy and transmission electron microscopy (TEM) were performed at different time points (1, 6, 24, 48 and 144 h) after MWCNT administration. The dynamics of oxidative stress parameters (malondialdehyde, protein carbonyls and reduced glutathione), along with nitrosative stress parameters (nitric oxide, inducible NO synthase), the pro-inflammatory cytokines [interleukin-(IL)-1β] and the number of cells expressing caspase 3 and proliferating cell nuclear antigen (PCNA) were assessed. Our results indicate that, after i.p. administration, MWCNT translocate progressively in the spleen, with a peak of concentration after 48 h, and determine lymphoid hyperplasia and an increase in the number of cells which undergo apoptosis, in parallel with the enhancement of the mitosis in the white pulp and with transient alterations of oxidative stress and inflammation that need further investigations for a longer period of monitoring.
机译：近年来，由于碳纳米管在纳米技术领域的潜在应用，大量研究集中在碳纳米管（CNT）的可能毒性上。脾毒性的研究是碳纳米管诱导的毒性评估的一部分。在这项研究中，我们研究了腹膜内（i.p.）给予单剂量[1.5 ml;每公斤体重（bw）2 mg的多壁（MW）碳纳米管（外径15-25 nm，内径10-15 nm，表面88 m2 g-1）按1：1的比例功能化-链DNA（ss-DNA-MWCNT，270 mg l-1）。用DNA进行的CNT功能化可确定在体液中的稳定分散。为了检测脾脏中的碳纳米管，在施用MWCNT后的不同时间点（1、6、24、48和144小时）进行了拉曼光谱，组织病理学检查，共聚焦显微镜和透射电子显微镜（TEM）。氧化应激参数（丙二醛，蛋白质羰基和还原型谷胱甘肽）的动力学，以及亚硝化应激参数（一氧化氮，诱导型一氧化氮合酶），促炎性细胞因子[白介素-（IL）-1β]和表达细胞的数量评估了半胱天冬酶3和增殖细胞核抗原（PCNA）。我们的结果表明，在i.p.给药后，MWCNT在脾脏中逐渐转移，在48小时后达到浓度峰值，并确定淋巴样增生和经历凋亡的细胞数量增加，同时白浆中的有丝分裂增强和短暂改变氧化应激和炎症的原因，需要进行更长时间的监测。

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