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首页> 外文期刊>Journal of applied toxicology >Preferential expression of matrix metalloproteinase-9 in mouse skin after sulfur mustard exposure.
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Preferential expression of matrix metalloproteinase-9 in mouse skin after sulfur mustard exposure.

机译：硫芥子气暴露后小鼠皮肤中基质金属蛋白酶9的优先表达。

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Matrix metalloproteinases (MMPs), a class of enzymes responsible for the degradation of extracellular matrix proteins, play important roles in inflammatory and immune responses. In skin, MMP-2 (gelatinase A) and MMP-9 (gelatinase B) are normally inactive but can be expressed during tissue injury. Both degrade collagen IV and other critical components of the basement membrane zone that separates the epidermis from the dermis. The expression of MMP-2 and -9 was studied in sulfur mustard (SM)-exposed ear skin from mice to determine their role in tissue vesicant injury. Punch biopsies of mouse ears were collected between 6 and 168 h after exposure to 97.5 mM (0.08 mg) SM diluted in CH(2)Cl(2). They were examined histologically and assayed for MMP-2 and -9 expression by gelatinase activity assays, real-time reverse transcriptase-polymerase chain reaction and Western blot analysis. A time-related increase in overall gelatinase activity was observed in SM-treated ears. At 168 h after SM exposure, the relative levels of MMP-9 mRNA were increased 27-fold and MMP-9 protein 9-fold when compared with the control (CH(2)Cl(2) treated) ears. In contrast, there were no observable increases in the MMP-2 mRNA or protein levels between treated and control ears. These observations suggest the differential expression of MMP-2 and -9 during the cutaneous response to SM injury and suggest a role for MMP-9 in SM-induced injury.
机译：基质金属蛋白酶（MMP）是负责细胞外基质蛋白降解的一类酶，在炎症和免疫反应中起重要作用。在皮肤中，MMP-2（明胶酶A）和MMP-9（明胶酶B）通常是无活性的，但可以在组织损伤期间表达。两者都会降解胶原蛋白IV和将表皮与真皮分开的基底膜区的其他关键成分。研究了MMP-2和-9在小鼠暴露于硫芥末（SM）的耳皮中的表达，以确定它们在组织表面泡沫损伤中的作用。暴露于在CH（2）Cl（2）中稀释的97.5 mM（0.08 mg）SM暴露后6至168 h之间，收集小鼠耳朵的打孔活检。对它们进行了组织学检查，并通过明胶酶活性测定，实时逆转录酶-聚合酶链反应和蛋白质印迹分析检测了MMP-2和-9的表达。在SM处理过的耳朵中观察到了总的明胶酶活性随时间的增加。与对照（CH（2）Cl（2）处理）的耳朵相比，SM暴露后168 h，MMP-9 mRNA的相对水平增加了27倍，MMP-9蛋白的相对水平增加了9倍。相反，在处理过的和对照的耳朵之间，MMP-2 mRNA或蛋白质水平没有明显增加。这些观察结果表明在皮肤对SM损伤的反应过程中MMP-2和-9的差异表达，并暗示了MMP-9在SM诱导的损伤中的作用。

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