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Effects of cylindrospermopsin on a common carp leucocyte cell line

机译:环精皮蛋白对鲤鱼白细胞的影响

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Cylindrospermopsin (CYN) is a cytotoxin produced by different cyanobacterial species, increasingly detected in water reservoirs worldwide. There is very little information available concerning the effects of the toxin on fish immune cells. The aim of the study was to elucidate the potential impact of cylindrospermopsin on the selected parameters of a common carp (Cyprinus carpio L.) leucocyte cell line (CLC). The cells were incubated with the cyanotoxin at concentrations of 10, 1 or 0.1 mu gml(-1) for up to 48h. Cell viability and proliferation, apoptosisecrosis induction, cell morphology and phagocytic activity were determined. The two higher toxin concentrations occurred to be evidently cytotoxic in a time-dependent manner and influenced all studied parameters. The lowest used concentration had no effects on cell viability and cell number; however, a strong reduction of bacteria uptake after 24-h exposure was detected. The obtained results indicate that cylindrospermopsin may interfere with the basic functions of fish phagocytic cells and as a consequence influence the fish immunity. Copyright (c) 2014 John Wiley & Sons, Ltd.
机译:Cylindrospermopsin(CYN)是由不同的蓝细菌产生的一种细胞毒素,在世界各地的水库中越来越多地被发现。关于毒素对鱼类免疫细胞影响的信息很少。这项研究的目的是阐明cylindrospermopsin对普通鲤鱼(Cyprinus carpio L.)白细胞细胞系(CLC)的选定参数的潜在影响。将细胞与浓度分别为10、1或0.1μgml(-1)的蓝藻毒素孵育48小时。测定细胞活力和增殖,凋亡/坏死诱导,细胞形态和吞噬活性。两种较高的毒素浓度以时间依赖性方式表现出明显的细胞毒性,并影响了所有研究参数。最低使用浓度对细胞活力和细胞数没有影响;但是,检测到24小时的接触后细菌的吸收大大降低。获得的结果表明,cylindrospermopsin可能会干扰鱼类吞噬细胞的基本功能,从而影响鱼类的免疫力。版权所有(c)2014 John Wiley&Sons,Ltd.

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