...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of applied toxicology >Local lymph node assay responses to paraphenylenediamine: intra- and inter-laboratory evaluations.
【24h】

Local lymph node assay responses to paraphenylenediamine: intra- and inter-laboratory evaluations.

机译：对苯二胺的局部淋巴结测定反应：实验室内和实验室间评估。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The murine local lymph node assay (LLNA) is a method for the prospective identification of skin sensitizing chemicals. Proliferative responses induced in lymph nodes draining the site of topical application of the test chemical are measured and those chemicals that induce a stimulation index of three or more compared with concurrent vehicle-treated controls are considered to have the potential to cause skin sensitization. Dose-response data from the LLNA may be used to derive an estimate of relative skin sensitizing potency, based upon derivation of the concentration of chemical required to cause a stimulation index of 3 (EC3 value) as calculated by linear interpolation. The purpose of the present investigations was to examine the stability of LLNA responses and the consistency of derived EC3 values induced by the contact allergen paraphenylenediamine (PPD). Analyses were conducted once a month over a 4-month period in each of two independent laboratories. In all assays, and in both laboratories, PPD elicited a positive response. Although some minor differences in responses between and within laboratories were observed, the derived EC3 values were generally very consistent. In Laboratory 1, EC3 values varied between 0.06 and 0.09% PPD, whereas in Laboratory 2 the range was 0.09-0.20%. These EC3 values are consistent with clinical experience of this material insofar as it is a common and relatively potent cause of allergic contact dermatitis in humans. Taken together, these data confirm the stability of LLNA responses both with time and between laboratories and provide additional support for the use of derived EC3 values in the assessment of relative skin sensitizing potency.
机译：鼠局部淋巴结测定法（LLNA）是一种前瞻性鉴定皮肤致敏化学物质的方法。测量在引流测试化学品局部应用部位的淋巴结中诱导的增殖反应，并且与同时用赋形剂处理的对照组相比，那些诱导刺激指数为3或更高的化学品被认为具有引起皮肤过敏的潜力。 LLNA的剂量反应数据可用于根据线性插值计算得出引起刺激指数为3（EC3值）所需的化学物质的浓度，得出相对的皮肤致敏能力的估计值。本研究的目的是检查LLNA反应的稳定性以及接触性过敏原对苯二胺（PPD）诱导的EC3值的一致性。在两个独立实验室中的每个实验室中，每月进行一次为期4个月的分析。在所有测定中，以及在两个实验室中，PPD均引起阳性反应。尽管观察到实验室之间和实验室之间的响应存在一些细微差异，但得出的EC3值通常非常一致。在实验室1中，EC3值在0.06-0.09％PPD之间变化，而在实验室2中，该范围为0.09-0.20％。这些EC3值与这种材料的临床经验一致，因为它是人类过敏性接触性皮炎的常见且相对有效的原因。综上所述，这些数据证实了LLNA反应随时间和实验室之间的稳定性，并为使用衍生的EC3值评估相对皮肤致敏力提供了额外的支持。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号