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首页> 外文期刊>Journal of applied toxicology >An improved brain slice model of nerve agent-induced seizure activity.
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An improved brain slice model of nerve agent-induced seizure activity.

机译：神经药引起的癫痫发作活动的改进的脑片模型。

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A brain slice model was developed to investigate the mechanisms of seizure activity induced by soman and the effectiveness of potential anticonvulsant drugs. Unlike previously reported slice studies with nerve agents, this model contains the entorhinal cortex as well as the hippocampus. This allows the study of the spread of seizure discharges within the limbic system and the development of prolonged, sustained discharges that are rarely seen in the simple hippocampal slice preparation. Soman (1 microM) induced a second population spike in the evoked field potential in the CA1 or CA3 region within 15-20 min. In almost all the slices tested, this developed into spontaneous seizure activity within 30-40 min. As well as interictal bursts, many slices also showed longer periods of high-frequency bursting analogous to ictal seizure activity that originated in the entorhinal cortex. This activity appeared similar to that induced by the muscarinic agonist pilocarpine. Both the second population spike and the spontaneous discharges could be blocked by diazepam and by AMPA/kainate antagonists, but not by the NMDA antagonists AP5 and MK-801. This study confirms that the combined hippocampal-entorhinal cortex slice preparation is a suitable model for investigating the origin and propagation of nerve-agent-induced seizures within the limbic system. Copyright 2001 John Wiley & Sons, Ltd.
机译：开发了脑切片模型以研究梭曼诱发癫痫发作的机制以及潜在的抗惊厥药物的有效性。与先前报道的对神经制剂的切片研究不同，该模型包含内嗅皮层和海马体。这可以研究癫痫发作放电在边缘系统内的分布以及长时间持续放电的发展，这在简单的海马切片制备中很少见。 Soman（1 microM）在15-20分钟内引起了CA1或CA3区域诱发的场电位的第二个峰值。在几乎所有测试的切片中，这在30-40分钟内发展为自发发作。除发作间期爆裂外，许多切片还显示出更长的高频爆发期，类似于源自内嗅皮层的发作性发作。该活性似乎类似于毒蕈碱激动剂毛果芸香碱诱导的活性。地西epa和AMPA /海藻酸酯拮抗剂均可阻断第二种群的峰值和自发放电，而NMDA拮抗剂AP5和MK-801则不能。这项研究证实，海马-肠胃皮质联合切片制备物是用于研究神经药诱发的边缘系统内癫痫发作的起源和传播的合适模型。版权所有2001 John Wiley＆Sons，Ltd.

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