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首页> 外文期刊>Journal of applied toxicology >Evaluation of genotoxic damage of cadmium chloride in peripheral blood of suckling Wistar rats.
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Evaluation of genotoxic damage of cadmium chloride in peripheral blood of suckling Wistar rats.

机译：评价哺乳Wistar大鼠外周血中氯化镉的遗传毒性。

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The aim of this study was to evaluate possible genotoxic damage of cadmium chloride exposure in suckling rats by means of the comet assay and the in vivo micronucleus test of rat blood lymphocytes, because no information is available on the genotoxic effect of cadmium in rats at this early age. Pups were receiving cadmium (as CdCl(2).H(2)O) orally in fractions of 0.5 mg for 9 days, totalling 4.5 mg Cd kg(-1) body wt, or were given a single subcutaneous injection of 0.5 mg Cd kg(-1) body wt. Some pups in both exposed groups were receiving calcium supplement (CaHPO(4).2H(2)O) in feed to reduce the body load of cadmium. Control pups did not receive either cadmium or calcium supplement. Cadmium in the carcass and organs was measured by atomic absorption spectrometry. The results showed that the cadmium body burden was significantly lower when the animals were receiving calcium supplements along with oral cadmium. The results of the micronucleus and comet assays showed significant differences between the control and exposed groups, regardless of the route of cadmium administration. The only statistically significant difference between the two exposed groups (oral cadmium and oral cadmium + calcium supplements) was in the number of micronuclei. The results of the comet assay showed that tail length differed statistically only between the control and all exposed groups, regardless of the route of cadmium administration. It can be concluded that the applied cadmium doses caused detectable genome damage but it was lower in calcium-treated pups receiving cadmium orally.
机译：这项研究的目的是通过彗星试验和大鼠血淋巴细胞的体内微核试验来评估乳鼠接触氯化镉的可能的遗传毒性损害，因为目前尚无关于镉对大鼠的遗传毒性作用的信息。早年。幼崽以0.5 mg的分数口服镉（作为CdCl（2）.H（2）O）9天，总计4.5 mg Cd kg（-1）体重，或皮下注射0.5 mg Cd kg（-1）体重两个暴露组中的某些幼崽都在饲料中添加钙补充剂（CaHPO（4）.2H（2）O）以减轻镉的身体负荷。对照幼犬未接受镉或钙补充。 atomic体和器官中的镉通过原子吸收光谱法测量。结果表明，当动物与口服镉一起服用钙补充剂时，镉的身体负担显着降低。微核和彗星试验的结果表明，对照组和裸露组之间的显着差异，与施用镉的途径无关。两个暴露组（口服镉和口服镉+钙补充剂）之间唯一的统计上显着差异是微核的数量。彗星试验的结果表明，无论镉施用途径如何，尾巴长度仅在对照组和所有暴露组之间具有统计学差异。可以得出结论，施用镉的剂量引起可检测的基因组损伤，但在口服镉的钙处理幼犬中较低。

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