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首页> 外文期刊>Journal of applied toxicology >Heterogeneity of CD80 gene transcription by human keratinocytes to allergens and irritants: relevance to allergic contact dermatitis in vivo.
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Heterogeneity of CD80 gene transcription by human keratinocytes to allergens and irritants: relevance to allergic contact dermatitis in vivo.

机译：人角质形成细胞对过敏原和刺激物CD80基因转录的异质性：与体内过敏性接触性皮炎的相关性。

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摘要

Using an in vitro assay for CD80 promoter activity, the transcriptional response of primary cultured human keratinocytes from different donors in response to allergens and irritants was studied. The CD80 promoter activity was increased reproducibly after exposure to certain chemicals. The epithelial cell lines HeLa and HaCaT also increased CD80 transcriptional activity in response to exposure to a panel of different allergens and irritants. Normal or immortalized keratinocytes responded reproducibly with a unique pattern to a panel of chemicals in which the culture increases CD80 transcription after exposure to certain chemicals and has no increase in CD80 transcription after exposure to other chemicals. Some keratinocyte cultures responded positively to certain chemicals whereas others did not respond to the same chemicals. Cultured keratinocytes from 16 of 20 adult donors (80%) responded to nitrochlorobenzene in vitro by increasing CD80 transcriptional activity, thus predicting subsequent sensitization to this same hapten (chi(2) = 7.2, P < 0.0073). Thus, a keratinocyte CD80 gene expression assay exhibits good sensitivity in predicting allergic contact dermatitis for sensitizers such as dinitrochlorobenzene. These data suggest that there is a rationale for developing the use of gene-transcription-based predictive assays to identify individual susceptibility to cutaneous reactivity after exposure to allergens and irritants. Copyright (c) 2004 John Wiley & Sons, Ltd.
机译：使用CD80启动子活性的体外测定，研究了来自不同供体的原代培养人角质形成细胞对过敏原和刺激物的转录反应。暴露于某些化学物质后，CD80启动子活性可再现地提高。上皮细胞系HeLa和HaCaT还响应暴露于一组不同的过敏原和刺激物而增加了CD80转录活性。正常或永生化的角质形成细胞以独特的模式可重复地响应一组化学物质，其中培养物在接触某些化学物质后增加CD80转录，而在接触其他化学物质后CD80转录没有增加。一些角质形成细胞培养物对某些化学物质呈阳性反应，而另一些对相同化学物质则无反应。来自20个成年供体中的16个（80％）的培养的角质形成细胞在体外通过增加CD80转录活性来响应硝基氯苯，从而预测随后对该相同的半抗原的致敏性（chi（2）= 7.2，P <0.0073）。因此，角质形成细胞CD80基因表达测定法在预测敏化剂（如二硝基氯苯）的过敏性接触性皮炎时表现出良好的敏感性。这些数据表明，开发基于基因转录的预测测定方法来鉴定暴露于过敏原和刺激物后个体对皮肤反应的敏感性是有理由的。版权所有（c）2004 John Wiley＆Sons，Ltd.

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