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首页> 外文期刊>Journal of assisted reproduction and genetics >Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes.
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Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes.

机译：体外培养中的干细胞因子/ c-Kit信号传导通过涉及Akt下游基因的机制加速增殖，从而支持早期小鼠胚胎发育。

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PURPOSE: stem cell factor (SCF)/c-Kit regulates the proliferation and survival of germ cells or stem cells; however, little is known about the role of SCF/c-Kit in pre-implantation embryo development. METHODS: using exogenous SCF supplementation and c-Kit siRNA injection, we investigated the role and mechanism of SCF/c-Kit in pre-implantation mouse embryos. RESULTS: addition of soluble SCF to the culture medium improved blastocyst formation. c-Kit gene silencing reduced the rate of blastocyst formation and delayed embryonic development. The number of proliferating cells in c-Kit gene-silenced blastocysts decreased, whereas the number of apoptotic cells in blastocysts obtained from both experimental and the control groups was not affected. RT-PCR, immunostaining and western blotting revealed that proliferation-related Akt downstream targets were substantially affected by c-Kit gene silencing. CONCLUSION: SCF/c-Kit signaling through Akt downstream targets is likely involved in mediating the cleavage and proliferation of blastomeres during mouse pre-implantation embryogenesis.
机译：目的：干细胞因子（SCF）/ c-Kit调节生殖细胞或干细胞的增殖和存活;然而，关于SCF / c-Kit在植入前胚胎发育中的作用知之甚少。方法：使用外源性SCF补充和c-Kit siRNA注射，我们研究了SCF / c-Kit在植入前小鼠胚胎中的作用和机制。结果：向培养基中添加可溶性SCF可改善胚泡的形成。 c-Kit基因沉默降低了胚泡形成的速度并延迟了胚胎发育。 c-Kit基因沉默的胚泡中增殖细胞的数量减少，而从实验组和对照组获得的胚泡中凋亡细胞的数量均不受影响。 RT-PCR，免疫染色和western blotting显示，与增殖相关的Akt下游靶标受c-Kit基因沉默的影响很大。结论：SCF / c-Kit信号通过Akt下游靶标参与小鼠胚胎植入前胚胎发生过程中可能介导卵裂球的分裂和增殖。

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