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首页> 外文期刊>Journal of assisted reproduction and genetics >Altitude can alter the mtDNA copy number and nDNA integrity in sperm.
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Altitude can alter the mtDNA copy number and nDNA integrity in sperm.

机译：海拔高度可以改变精子中的mtDNA拷贝数和nDNA完整性。

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OBJECTIVE: Mitochondria are factories for energy production and genetic alterations in mtDNA will directly impact OXPHOS function. The copy number of mtDNA (i.e., the number of mtDNA per spermatozoon) is one of the major mitochondrial genetic features. Besides mtDNA copy number, the change of either mtDNA or nDNA integrity is another important factor causing asthenospermia, or poor sperm motility in infertile men. In this study, we investigated the mtDNA copy number and the integrities of mtDNA and nDNA respectively in semen samples from different donors at 5,300 m altitudes. METHODS: Total DNA was extracted from semen samples from donors in two different altitudes. Quantitative PCR was performed to evaluate the mtDNA copy number. PCR amplification was used to examine the integrity of sperm mtDNA. Flow cytometry was carried out to investigate sperm nDNA integrity. All data were analyzed to show the statistical significance. RESULTS: Sperm mtDNA copy number for those living at high altitude (5,300 m) for one month was significantly higher (P < 0.05) than for those at the lower altitude (1,400 m) or in donors who had been living at the 5,300 m altitude for 1 year. In addition, sperm mtDNA copy numbers were remarkably decreased (P < 0.05) in those who had lived at the greater altitude for 1 year compared to those who had lived there for one month. The ratio of nDNA integrity among the 10,000 sperms at high altitude for one month was significantly lower (P < 0.05) than that at the lower altitude (1,400 m) or at 5,300 m for 1 year, and the ratio of nDNA integrity sperms at high altitude for 1 year was increased, and higher than that for at the lower altitude (P < 0.05). CONCLUSION: High altitude can alter the mtDNA copy number and nDNA integrity in the sperms.
机译：目的：线粒体是生产能量的工厂，mtDNA的遗传改变将直接影响OXPHOS的功能。 mtDNA的拷贝数（即每个精子的mtDNA数）是主要的线粒体遗传特征之一。除了mtDNA拷贝数外，mtDNA或nDNA完整性的变化是导致不育症的精子运动弱或精子活动力差的另一个重要因素。在这项研究中，我们调查了5300 m高度不同供体的精液样品中mtDNA的拷贝数和mtDNA和nDNA的完整性。方法：从两个不同高度的供体的精液样本中提取总DNA。进行定量PCR以评估mtDNA拷贝数。 PCR扩增用于检查精子mtDNA的完整性。进行流式细胞术以研究精子nDNA的完整性。分析所有数据以显示统计学意义。结果：高海拔地区（5,300 m）生活一个月的人的精子mtDNA拷贝数显着高于低海拔地区（1,400 m）或生活在5,300 m海拔的供者的精子mtDNA拷贝数（P <0.05）一年。此外，与在高海拔地区生活1个月的人相比，在高海拔地区生活1年的人的精子mtDNA拷贝数显着减少（P <0.05）。高海拔1万个精子中1个月的nDNA完整性比率显着低于低海拔（1,400 m）或5300 m处1年的nDNA完整性比率（P <0.05） 1年的海拔升高了，并且比低海拔的海拔更高（P <0.05）。结论：高海拔会改变精子的mtDNA拷贝数和nDNA完整性。

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