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首页> 外文期刊>Journal of Basic Microbiology: An International Journal on Morphology, Physiology, Genetics, and Ecology of Microorganisms >Anoxybacillus salavatliensis sp. nov., an alpha-glucosidase producing, thermophilic bacterium isolated from Salavatli, Turkey.
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Anoxybacillus salavatliensis sp. nov., an alpha-glucosidase producing, thermophilic bacterium isolated from Salavatli, Turkey.

机译：salavatliensis厌氧杆菌nov。，一种产生α-葡萄糖苷酶的嗜热细菌，从土耳其的萨拉瓦特利分离。

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摘要

A novel moderately thermophilic, Gram-positive staining, rod-shaped, spore-forming, motile, facultative anaerobic, and alpha-glucosidase producing strain A343(T), was isolated from a high temperature well-pipeline sediment sample in Salavatli province of Aydin, Turkey. Growth was observed at 37-69 masculineC (optimum 60 degrees C), at pH 5.5-9.5 (optimum 8.0-9.0) and at salinities from 0 to 4.5% (w/v) (optimum 2%). Strain A343(T) was able to grow on a wide range of carbon sources. Gelatin and starch utilization, amylase, catalase and oxidase activities, reduction of nitrate to nitrite were all positive. The G+C content of the genomic DNA was 45.1 mol%. The major menaquinone was MK-7. The dominant cellular fatty acids were: iso-C15:0, C16:0, and iso-C17:0. The phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain A343(T) belonged to the genus Anoxybacillus. The 16S rRNA gene sequence similarity between strain A343(T) and the type strains of recognized Anoxybacillus species was ranged from 95.8 to 99.4%. DNA-DNA hybridization revealed low homology with its closest relative Anoxybacillus kamchatkensis (49.7%). In addition to the total cell protein profiles, the Rep-PCR and the intergenic 16S-23S rRNA gene fingerprinting profiles differentiated strain A343(T) from all of the reference Anoxybacillus species used. Based upon phenotypic, phylogenetic and chemotaxonomic evidence, strain A343(T) was assigned to a new species within the genus Anoxybacillus, A. salavatliensis sp. nov. (The type strain A343(T) = DSM 22626(T) = NCIMB 14579(T)). The 16S rRNA gene nucleotide sequence of strain A343(T) is available in the GenBank database under the accession number--EU326496. Weinheim).
机译：从Aydin的Salavatli省的高温井管道沉积物样品中分离出了新型的适度嗜热，革兰氏阳性染色，棒状，孢子形成，运动，兼性厌氧和产生α-葡萄糖苷酶的菌株A343（T）。 ， 火鸡。在37-69℃（最适度60摄氏度），pH 5.5-9.5（最适度8.0-9.0）和盐度为0至4.5％（w / v）（最适2％）下观察到生长。菌株A343（T）能够在多种碳源上生长。明胶和淀粉利用率，淀粉酶，过氧化氢酶和氧化酶活性，硝酸盐还原为亚硝酸盐均呈阳性。基因组DNA的G + C含量为45.1mol％。主要的甲萘醌为MK-7。占优势的细胞脂肪酸为：iso-C15：0，C16：0和iso-C17：0。根据16S rRNA基因序列的系统发育分析表明，菌株A343（T）属于无氧杆菌属。 A343（T）菌株和已识别的厌氧杆菌属类型菌株之间的16S rRNA基因序列相似性范围为95.8％至99.4％。 DNA-DNA杂交显示出与其最接近的亲戚堪萨克厌氧杆菌（Anoxybacillus kamchatkensis）同源性低（49.7％）。除了总细胞蛋白谱外，Rep-PCR和基因间16S-23S rRNA基因指纹图谱还使菌株A343（T）与所使用的所有参考无氧杆菌属物种区分开。根据表型，系统发育和化学分类学证据，将菌株A343（T）分配给无氧杆菌属A. salavatliensis sp。的一个新物种。十一月（类型菌株A343（T）= DSM 22626（T）= NCIMB 14579（T））。 A343（T）菌株的16S rRNA基因核苷酸序列可从GenBank数据库中获得，登录号为--EU326496。温海姆）。

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