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首页> 外文期刊>Journal of biological inorganic chemistry: JBIC: a publication of the Society of Biological Inorganic Chemistry >A novel tool for capture and detection of typhoid fever using Ag-labeled nanocomposites
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A novel tool for capture and detection of typhoid fever using Ag-labeled nanocomposites

机译：使用Ag标记的纳米复合材料捕获和检测伤寒的新型工具

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This work investigated a simple and versatile modification to a solid substrate to develop antibody recognition using nanoparticles. The new immobilized metal ion affinity adsorbent containing nanoparticles and hydrophilic resins is proposed here to improve the binding of antigen on its surface. The light-scattering properties of submicroscopic metal particles ranging from 100 to 120 nm in diameter were confirmed by scanning electron microscopy. We found that synthesized nanoparticles have an inherent enzyme mimetic activity similar to that found in natural peroxidases. The synthesized nanoparticles were coated with Salmonella typhi and Salmonella paratyphi antigens and were allowed to react with Salmonella-infected serum. Positive reactions were detected visually with the naked eye. The color changes of substrate (TMB) were observed even in 1:800 dilutions. This report helps in developing a specific immunoassay using nano-conjugated antigen for the rapid detection of S. typhi and S. paratyphi antibodies in infected serum.
机译：这项工作研究了对固体基质的简单通用修饰，以开发使用纳米颗粒的抗体识别。本文提出了一种新型的包含纳米粒子和亲水性树脂的固定化金属离子亲和吸附剂，以改善抗原在其表面上的结合。通过扫描电子显微镜确认直径在100至120nm范围内的亚显微金属颗粒的光散射特性。我们发现合成的纳米粒子具有与天然过氧化物酶相似的固有酶模拟活性。合成的纳米粒子被伤寒沙门氏菌和副伤寒沙门氏菌抗原包被，并使其与沙门氏菌感染的血清反应。用肉眼目测检测到阳性反应。甚至在1：800的稀释液中也观察到底物（TMB）的颜色变化。该报告有助于开发使用纳米共轭抗原的特异性免疫测定法，以快速检测感染血清中的伤寒沙门氏菌和副伤寒沙门氏菌抗体。

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