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首页> 外文期刊>Journal of biological rhythms >Immunoreactivities to three circadian clock proteins in two ground crickets suggest interspecific diversity of the circadian clock structure
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Immunoreactivities to three circadian clock proteins in two ground crickets suggest interspecific diversity of the circadian clock structure

机译：在两个地面中对三种生物钟蛋白的免疫反应性表明生物钟结构的种间多样性

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摘要

The closely related crickets Dianemobius nigrofasciatus and Allonemobius allardi exhibit similar circadian rhythms and photoperiodic responses, suggesting that they possess similar circadian and seasonal clocks. To verify this assumption, antisera to Period (PER), Doubletime (DBT), and Cryptochrome (CRY) were used to visualize circadian clock neurons in the cephalic ganglia. Immunoreactivities referred to as PER-ir, DBT-ir, and CRY-ir were distributed mainly in the optic lobes (OL), pars intercerebralis (PI), dorsolateral protocerebrum, and the subesophageal ganglion (SOG). A system of immunoreactive cells in the OL dominates in D. nigrofasciatus, while immunoreactivities in the PI and SOG prevail in A. allardi. Each OL of D. nigrofasciatus contains 3 groups of cells that coexpress PER-ir and DBT-ir and send processes over the frontal medulla face to the inner lamina surface suggesting functional linkage to the compound eye. Only 2 pairs of PER-ir cells (no DBT-ir) were found in the OL of A. allardi. Several groups of PER-ir cells occur in the brain of both species. The PI also contains DBT-ir and CRY-ir cells, but in A. allardi, most of the DBT-ir is confined to the SOG. Most immunoreactive cells in the PI and in the dorsolateral brain send their fibers to the contralateral corpora cardiaca and corpora allata. The proximity and, in some cases, proven identity of the PER-ir, DBT-ir, and CRY-ir perikarya are consistent with presumed interactions between the examined clock components. The antigens were always found in the cytoplasm, and no diurnal oscillations in their amounts were detected. The photoperiod, which controls embryonic diapause, the rate of larval development, and the wing length of crickets, had no discernible effect on either distribution or the intensity of the immunostaining.
机译：密切相关的Dianemobius nigrofasciatus和Allonemobius allardi表现出相似的昼夜节律和光周期反应，表明它们具有相似的昼夜节律和季节性时钟。为了验证此假设，使用抗血清（PER），双倍时间（DBT）和隐色（CRYchrome）的抗血清来可视化头神经节中的昼夜节律时钟神经元。称为PER-ir，DBT-ir和CRY-ir的免疫反应性主要分布在视神经叶（OL），大脑皮层pars（PI），背外侧前脑和食管下神经节（SOG）中。 OL中的一种免疫反应性细胞系统在黑纹线虫中占主导地位，而PI和SOG中的免疫反应性在阿拉德曲霉中占主导地位。黑腹果蝇的每个OL包含3组细胞，它们共表达PER-ir和DBT-ir，并在额髓面至内层薄层表面发送过程，提示与复眼的功能连接。在A. allardi的OL中仅发现2对PER-ir细胞（无DBT-ir）。两种动物的大脑中都存在几组PER-ir细胞。 PI还包含DBT-ir和CRY-ir细胞，但是在A. allardi中，大多数DBT-ir都局限于SOG。 PI和背外侧脑中的大多数免疫反应性细胞将其纤维发送至对侧cardiac门体和异体体。 PER-ir，DBT-ir和CRY-ir核果的亲近性（在某些情况下还被证明是其身份）与所检查时钟组件之间的假定相互作用相一致。总是在细胞质中发现抗原，并且没有检测到其数量的昼夜振荡。控制胚胎滞育，幼虫发育的速度和the的翼长的光周期对免疫染色的分布或强度没有明显的影响。

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