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首页> 外文期刊>Journal of Beijing forestry University >Establishment of Embryogenic Cell Suspension Cultures and Development of Somatic Embryo onCamellia sinensis var. assamica Kitamura I. Establishment of Embryogenic Suspension Cell Line
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Establishment of Embryogenic Cell Suspension Cultures and Development of Somatic Embryo onCamellia sinensis var. assamica Kitamura I. Establishment of Embryogenic Suspension Cell Line

机译：茶树胚细胞悬浮培养的建立及体细胞胚的发育Assamica Kitamura I.胚胎发生悬浮细胞系的建立

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Calli derived from mature cotyledon of C. sinensis var. assamica Kitamura were subcultured in the modified MS' agar medium supplement with 0. 05mg.l~(-1)2,4-D and 0. 5mg.l~(-1) 6-BA. Various kinds of calli were formed after many subcultures, includingyellow friable callus (YF) , yellow semi-transparent callus (YS), green semi-transparent callus (GS), green hard callus (GH) and white hard callus (WH). The friability of calli and components of the medium affecting the establishment of cell suspensionculture were tested; (1) YF was the most effective one; (2) Better growth and separation of cells was achieved when the cells were grown in the modified MS' liquid medium with higher vitamin levels (18. 03mg.l~(-1)) and a higher ratio of auxin:cytokine (2,4-D: 6-BA= 0. 1:0. 5mg.l~(-1)) plus coconut milk(20ml.l~(-1)). The embroygenic cell suspension culture was established using YF and the modified MS' liquid medium with characteristics of 5. 36% single cell, 23. 81% 2 — 5 cells aggregates and with 70.83% 6 — 20 cells aggregates with 70% —80% lively cells and 3. 25% mitosis coefficient after 3 weeks incubation under diffuse light delivered during a 16hr day at 25 + 2 deg C on 120rpm shaker. The cell growth period of the suspension culture was 10 days or so.
机译：中华C子叶成熟子叶的愈伤组织。在改良的MS'琼脂培养基补充液中以0. 05mg.l〜（-1）2,4-D和0. 5mg.l〜（-1）6-BA继代培养assamica Kitamura。经过许多次培养后形成了各种愈伤组织，包括黄色脆性愈伤组织（YF），黄色半透明愈伤组织（YS），绿色半透明愈伤组织（GS），绿色硬愈伤组织（GH）和白色硬愈伤组织（WH）。测试了愈伤组织的易碎性和影响细胞悬浮培养的培养基成分。 （1）YF是最有效的一种; （2）当细胞在维生素水平较高（18. 03mg.l〜（-1））和生长素：细胞因子比例较高的改良MS'液体培养基中生长时，细胞生长和分离效果更好（2， 4-D：6-BA = 0。1：0。5mg.l〜（-1））加椰奶（20ml.l〜（-1））。使用YF和改良的MS'液体培养基建立胚胎发生细胞悬浮培养物，其特征为5。36％的单细胞，23。81％的2-5细胞聚集体和70.83％的6-20的细胞聚集体与70％-80％在120 rpm摇床中于25 + 2摄氏度的条件下，在16小时的一天中于16小时内递送的漫射光下孵育3周后，在3周内培养了活细胞和3. 25％的有丝分裂系数。悬浮培养的细胞生长期为10天左右。

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