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首页> 外文期刊>Journal of biochemical and molecular toxicology >Ethanol-induced alterations of the antioxidant defense system in rat kidney.
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Ethanol-induced alterations of the antioxidant defense system in rat kidney.

机译：乙醇诱导的大鼠肾脏抗氧化防御系统改变。

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We report here the effects of chronic ethanol consumption on the antioxidant defense system in rat kidney. Thirty-two male Wistar rats were randomly divided in two identical groups and were treated as follows: control group (water for fluid) and the ethanol-fed group (2 g/kg body weight/24 h). The animals were sacrificed after 10 weeks, and respectively 30 weeks of ethanol consumption, and the renal tissue was isolated and analyzed. Results revealed that kidney alcohol dehydrogenase activities increased significantly after ethanol administration, but the electrophoretic pattern of alcohol dehydrogenase isoforms was unmodified. The SDS polyacrylamidegel electrophoretic study of kidney proteins has revealed the appearance of two new protein bands after long-term ethanol consumption. The kidney reduced glutathione/oxidized glutathione ratio decreased, indicating an oxidative stress response due to ethanol ingestion. The malondialdehyde contents and xanthine oxidase activities were unchanged. The antioxidant enzymatic defense system showed a different response during the two periods of ethanol administration. After 10 weeks, catalase, glutathione peroxidase, glutathione reductase, and glucose-6-phosphate dehydrogenase were activated, while superoxide dismutase, glutathione transferase, and gamma-glutamyltranspeptidase levels were stationary. After 30 weeks, superoxide dismutase and glutathione peroxidase activities were unmodified, but catalase, glutathione transferase, gamma-glutamyltranspeptidase, glutathione reductase, and glucose-6-phosphate dehydrogenase activities were significantly increased. Remarkable changes have been registered after 30 weeks of ethanol administration for glutathione reductase and glucose-6-phosphate dehydrogenase activities, including an increase by 106 and 216' of control values, respectively. These results showed specific changes in rat kidney antioxidant system and glutathione status as a consequence of long-term ethanol administration.
机译：我们在这里报告慢性乙醇消耗对大鼠肾脏抗氧化防御系统的影响。将32只雄性Wistar大鼠随机分为两个相同的组，并按以下方法治疗：对照组（液体换水）和乙醇喂养的组（2 g / kg体重/ 24 h）。在消耗乙醇10周和30周后分别处死动物，并分离和分析肾脏组织。结果显示，乙醇给药后，肾脏酒精脱氢酶活性显着增加，但酒精脱氢酶同工型的电泳模式未改变。肾脏蛋白的SDS聚丙烯酰胺凝胶电泳研究表明，长期喝乙醇后出现了两个新的蛋白条带。肾脏减少的谷胱甘肽/氧化型谷胱甘肽比率降低，表明由于摄入乙醇而产生氧化应激反应。丙二醛含量和黄嘌呤氧化酶活性未改变。抗氧化剂的酶促防御系统在乙醇施用的两个期间显示出不同的响应。 10周后，过氧化氢酶，谷胱甘肽过氧化物酶，谷胱甘肽还原酶和6-磷酸葡萄糖脱氢酶被激活，而超氧化物歧化酶，谷胱甘肽转移酶和γ-谷氨酰转肽酶水平则保持稳定。 30周后，超氧化物歧化酶和谷胱甘肽过氧化物酶活性未改变，但过氧化氢酶，谷胱甘肽转移酶，γ-谷氨酰转肽酶，谷胱甘肽还原酶和葡萄糖6-磷酸脱氢酶活性显着增加。乙醇施用30周后，谷胱甘肽还原酶和6-磷酸葡萄糖脱氢酶的活性发生了显着变化，其中控制值分别增加了106和216'。这些结果表明，长期服用乙醇会导致大鼠肾脏抗氧化系统和谷胱甘肽状态发生特定变化。

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