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首页> 外文期刊>Journal of Bioactive and Compatible Polymers >Effect of Surface-activated PLLA Scaffold on Apatite Formation in Simulated Body Fluid
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Effect of Surface-activated PLLA Scaffold on Apatite Formation in Simulated Body Fluid

机译：表面活化的PLLA支架对模拟体液中磷灰石形成的影响

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摘要

Surface-activated poly(L-lactic acid) (PLLA) films and scaffolds were investigated for their effect on the formation of hydroxyapatite (HA) in simulated body fluid (SBF). PLLA samples were treated with plasma discharge in oxygen gas; the activated polymer surfaces were subjected to in situ grafting acrylic acid (AA) monomer. The obtained PLLA-PAA was converted to PLLA-PAA-HA in SBF. The formation of HA crystals was identified by surface analyses and the size and distribution by scanning electron miscroscopy. The major elements of HA surface-modified PLLA were confirmed by electron spectroscopy for chemical analysis and attenuated total reflectance-Fourier transform infrared spectra. Fibroblast, chondrocyte, and osteoblast cells were seeded in scaffolds and cultivated in vitro; the total cellularity was higher in the PLLA-PAA-HA scaffolds than the PLLA and PLLA-HA. Histological staining of the cells was denser in the cell-seeded PLLA-PAA-HA constructs. The introduction of specific functionality on the polymer surface significantly improved apatite nucleation and growth. Thus, HA-formed PLLA scaffolds are potentially useful in musculoskeletal tissue engineering.
机译：研究了表面活化的聚L-乳酸（PLLA）膜和支架对模拟体液（SBF）中羟基磷灰石（HA）形成的影响。 PLLA样品在氧气中通过等离子体放电处理;使活化的聚合物表面原位接枝丙烯酸（AA）单体。在SBF中将获得的PLLA-PAA转化为PLLA-PAA-HA。 HA晶体的形成通过表面分析确定，尺寸和分布通过扫描电子显微镜观察。 HA表面改性的PLLA的主要元素已通过电子光谱法进行了化学分析，并且衰减了全反射-傅立叶变换红外光谱。将成纤维细胞，软骨细胞和成骨细胞接种在支架上并进行体外培养; PLLA-PAA-HA支架的总细胞数高于PLLA和PLLA-HA。细胞的组织学染色在接种了细胞的PLLA-PAA-HA构建体中更为密集。在聚合物表面上引入特定官能团可显着改善磷灰石的成核和生长。因此，HA形成的PLLA支架可能在肌肉骨骼组织工程中有用。

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