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首页> 外文期刊>Journal of biochemical and molecular toxicology >Differential toxicities of air (mO-LDL) or copper-oxidized LDLs (Cu-LDL) toward endothelial cells.
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Differential toxicities of air (mO-LDL) or copper-oxidized LDLs (Cu-LDL) toward endothelial cells.

机译：空气（mO-LDL）或铜氧化的LDL（Cu-LDL）对内皮细胞的毒性不同。

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In vivo low density protein (LDL) oxidation is a progressive phenomenon leading to the presence of minimally and highly oxidized LDLs in the subendothelial arterial space. Oxidized LDLs have been reported to be cytotoxic against endothelial cells. The goal of this study was to determine which of the minimally and highly oxidized LDLs were the most cytotoxic against bovine aortic endothelial cells (BAEC). Both the morphological aspect of the cells themselves, and LDH or MTT tests revealed that mO- or Cu-LDLs had similar cytotoxicity with up to 8 hours of oxidation, showing no relation with the level of LDL oxidation; for longer oxidation times, Cu-LDL cytotoxicity decreased. This phenomenon is linked to their different oxidation kinetics. Moreover, in the initial hours following BAEC incubation with mO- or Cu-LDLs, total cell glutathione dropped, whereas after 16 hours of incubation, highly oxidized Cu-LDL increased the glutathione level in the cell. The biphasic evolution of glutathione concentration corresponds to an autoprotective mechanism of cells against oxidized LDL cytotoxicity. This study suggests that the specific chemical characteristics of the different types of oxidized LDLs should always be precisely described in future assays devoted to studying the biological effects of what are known under the generic term as "oxidized LDLs". This precaution should prevent any confusion in interpreting different studies.
机译：体内低密度蛋白（LDL）氧化是一种渐进现象，导致内皮下动脉间隙中存在最低限度和高度氧化的LDL。据报道氧化的LDL对内皮细胞具有细胞毒性。这项研究的目的是确定哪些最低限度和高度氧化的LDL对牛主动脉内皮细胞（BAEC）最具细胞毒性。细胞本身的形态和LDH或MTT测试都表明，mO-或Cu-LDLs在长达8小时的氧化过程中具有相似的细胞毒性，与LDL氧化水平无关。对于更长的氧化时间，Cu-LDL细胞毒性降低。这种现象与它们不同的氧化动力学有关。此外，在与mO-或Cu-LDLs进行BAEC孵育后的最初几个小时，总细胞谷胱甘肽下降，而在孵育16小时后，高度氧化的Cu-LDL增加了细胞中的谷胱甘肽水平。谷胱甘肽浓度的双相演变对应于细胞抗氧化LDL细胞毒性的自我保护机制。这项研究表明，在以后的研究中，应始终精确地描述不同类型的氧化LDL的特定化学特征，这些研究专门研究通用术语“氧化LDL”的生物效应。这种预防措施应防止在解释不同研究时出现任何混淆。

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