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首页> 外文期刊>Journal of biochemical and molecular toxicology >Madecassoside, a triterpenoid saponin isolated from Centella asiatica herbs, protects endothelial cells against oxidative stress
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Madecassoside, a triterpenoid saponin isolated from Centella asiatica herbs, protects endothelial cells against oxidative stress

机译：积雪草苷，一种从积雪草中分离的三萜皂苷，可保护内皮细胞免受氧化应激

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摘要

This study aimed to investigate the effect of madecassoside against oxidative stress-induced injury of endothelial cells. Hydrogen peroxide (H 2O 2, 500 μmol/L) was employed as an inducer of oxidative stress in human umbilical vein endothelial cells (HUVECs). Cell apoptosis was detected by Hoechst 33258 staining and flow cytometry. Caspase-3 activity and mitochondria membrane potential were further examined. As a result, madecassoside (10, 30, 100 μmol/L) could reverse morphological changes, elevate cell viability, increase glutathione levels, and decrease lactate dehydrogenase and malondialdehyde levels caused by H 2O 2 in a concentration-dependent manner. It attenuated apoptosis, preventing the activation of caspase-3 and the loss of mitochondria membrane potential, as well as the phosphorylation of p38 mitogen-activated protein kinase (MAPK) in HUVECs. These data suggested that madecassoside could protect HUVECs from oxidative injury, which was probably achieved by inhibiting cell apoptosis via protection of mitochondria membranes and downregulation of the activation of caspase-3 and p38 MAPK.
机译：这项研究旨在调查马卡德西苷对氧化应激诱导的内皮细胞损伤的影响。过氧化氢（H 2O 2，500μmol/ L）被用作人脐静脉内皮细胞（HUVEC）中氧化应激的诱导剂。 Hoechst 33258染色和流式细胞仪检测细胞凋亡。进一步检查了Caspase-3活性和线粒体膜电位。结果，马卡德糖苷（10、30、100μmol/ L）可以以浓度依赖的方式逆转由H 2O 2引起的形态变化，提高细胞活力，增加谷胱甘肽水平并降低乳酸脱氢酶和丙二醛水平。它减弱了细胞凋亡，阻止了caspase-3的激活和线粒体膜电位的丧失，以及HUVEC中p38丝裂原活化蛋白激酶（MAPK）的磷酸化。这些数据表明，羟基积雪草苷可以保护HUVEC免受氧化损伤，这可能是通过保护线粒体膜并下调caspase-3和p38 MAPK的活化来抑制细胞凋亡来实现的。

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